Inhibition of selectin-dependent tumor cell adhesion to endothelial cells and platelets by blocking O-glycosylation of these cells.

Abstract

Expression of sialosyl-Le(x) (SLe(x)) and sialosyl-Le(a) (SLe(a)) on tumor cell lines HL60, Colo205, and U937 was greatly suppressed by application of benzyl-alpha-GalNAc for inhibition of O-linked carbohydrate chain extension, which resulted in reduced adhesion of tumor cells to activated endothelial cells or platelets mediated by ELAM-1 (E-selectin) or GMP-140 (P-selectin). Inhibitors or modifiers of N-glycosylation had no effect on expression of SLe(x) or SLe(a) in these tumor cells. These findings suggest the possibility that targeting of O-glycosylation inhibitors or modifiers to tumor cells may effectively suppress metastatic potential.

• Authors: N Kojima, K Handa, W Newman, S Hakomori
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 182 Issue 3 Pg. 1288-95 (Feb 14 1992) ISSN: 0006-291X [Print] United States
• PMID: 1371678 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Monoclonal
• Cell Adhesion Molecules
• E-Selectin
• Lewis Blood Group Antigens
• P-Selectin
• Platelet Membrane Glycoproteins
• Acetylgalactosamine

Topics

• Acetylgalactosamine (analogs & derivatives)
• Antibodies, Monoclonal
• Blood Platelets (physiology)
• Cell Adhesion
• Cell Adhesion Molecules (physiology)
• Cell Line
• Cells, Cultured
• Colonic Neoplasms
• E-Selectin
• Endothelium, Vascular (physiology)
• Flow Cytometry
• Glycosylation
• Humans
• Leukemia, Promyelocytic, Acute
• Lewis Blood Group Antigens (physiology)
• Lymphoma, Large B-Cell, Diffuse
• P-Selectin
• Platelet Membrane Glycoproteins (physiology)