Abstract |
We previously proposed that intracellular cyclic AMP accumulation induces a putative, rapidly turning over protein inhibitory to further hormone activation of adenylate cyclase. In the present study, 2-aminopurine, which has been reported to selectively block c-fos gene expression, was used to test the hypothesis that c-fos protein might be involved in the desensitization to catecholamines was observed in 2-aminopurine-treated C6-2B rat glioma cells. However, we found 2-aminopurine to inhibit, in a concentration-dependent manner, total cellular RNA and protein synthesis in C6-2B, HeLa, Swiss 3T3 and BALB/c cells. mRNA synthesis was also markedly reduced in 2-aminopurine-treated cells. These unexpected findings, while supporting our hypothesis of a protein synthesis-sensitive step in the development of refractoriness, raise concern about the specificity of action of 2-aminopurine to inhibit c-fos induction and thus any cellular process, including desensitization, which might be regulated by c-fos gene expression.
|
Authors | M A DeBernardi, G Brooker |
Journal | FEBS letters
(FEBS Lett)
Vol. 296
Issue 1
Pg. 112-6
(Jan 13 1992)
ISSN: 0014-5793 [Print] England |
PMID | 1370421
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
|
Chemical References |
- Catecholamines
- Protein Synthesis Inhibitors
- Proto-Oncogene Proteins c-fos
- RNA, Messenger
- Colforsin
- 2-Aminopurine
- RNA
- Bucladesine
- Methionine
- Cyclic AMP
- Isoproterenol
- Uridine
|
Topics |
- 2-Aminopurine
(pharmacology)
- 3T3 Cells
(drug effects)
- Animals
- Bucladesine
(pharmacology)
- Catecholamines
(metabolism)
- Colforsin
(pharmacology)
- Cyclic AMP
(metabolism)
- Drug Interactions
- Gene Expression
(drug effects)
- Glioma
- HeLa Cells
(drug effects)
- Humans
- Isoproterenol
(pharmacology)
- Methionine
(metabolism)
- Mice
- Mice, Inbred BALB C
- Protein Biosynthesis
- Protein Synthesis Inhibitors
(pharmacology)
- Proto-Oncogene Proteins c-fos
(genetics, metabolism)
- RNA
(biosynthesis)
- RNA, Messenger
(drug effects, metabolism)
- Rats
- Tumor Cells, Cultured
(drug effects)
- Uridine
(metabolism)
|