Abstract | BACKGROUND: In mouse, IgG1 has been reported to make up 2 functionally distinct phenotypes that also differ in their induction requirements. One of these phenotypes lacks anaphylactic activity. OBJECTIVE: METHODS: Mice were immunized with allergen-encoding plasmid DNA or with recombinant allergens and alum. Sera were analyzed for IgG subclasses by ELISA for anaphylactic IgE by rat basophil degranulation, and after heat inactivation of IgE for anaphylactic IgG by passive cutaneous anaphylaxis assay. IFN-gamma and IL-5 from in-vitro restimulated spleen cells were quantitated by ELISA. RESULTS: After protein immunization, mice produced IgG1 and IgE, whereas DNA immunization elicited IgG1 and IgG2a but no IgE. However, all sera were positive for non- IgE-mediated passive cutaneous anaphylaxis. In the presence of anaphylactic IgG1, the additional occurrence of nonanaphylactic IgG1 cannot be strictly ruled out. To circumvent this problem, we immunized IL-4 receptor-deficient mice against Bet v 1a, because anaphylactic but not nonanaphylactic IgG1 has been reported to depend on IL-4. These animals produced only low amounts of IgG1, but sera were again positive for non- IgE-mediated anaphylactic activity. CONCLUSIONS: Our results revealed no evidence for the production of nonanaphylactic IgG1. Furthermore, our data indicate that the development of non- IgE-mediated anaphylaxis does not require IL-4 receptor signaling.
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Authors | Romana Hochreiter, Fatima Ferreira, Josef Thalhamer, Peter Hammerl |
Journal | The Journal of allergy and clinical immunology
(J Allergy Clin Immunol)
Vol. 112
Issue 3
Pg. 579-84
(Sep 2003)
ISSN: 0091-6749 [Print] United States |
PMID | 13679818
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Allergens
- Antigens, Plant
- Api g 1 protein, Apium graveolens
- Cytokines
- Immunoglobulin G
- Plant Proteins
- Receptors, Interleukin-4
- Vaccines, DNA
- Bet v 1 allergen, Betula
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Topics |
- Allergens
(administration & dosage, genetics)
- Anaphylaxis
(etiology, immunology, therapy)
- Animals
- Antigens, Plant
- Cell Degranulation
- Cytokines
(biosynthesis)
- Female
- Immunization
- Immunoglobulin G
(blood)
- Immunotherapy
- In Vitro Techniques
- Mice
- Mice, Inbred BALB C
- Phenotype
- Plant Proteins
(administration & dosage, genetics)
- Receptors, Interleukin-4
(metabolism)
- Signal Transduction
- Th1 Cells
(immunology)
- Tumor Cells, Cultured
- Vaccines, DNA
(genetics, immunology, pharmacology)
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