Protection against detrimental effects of potyvirus infection in transgenic tobacco plants expressing the papaya ringspot virus coat protein gene.

Abstract

We obtained transgenic tobacco plants expressing the papaya ringspot virus (PRV) coat protein (CP) gene by transformation via Agrobacterium tumefaciens. Expression was effectively monitored by enzyme-linked immunosorbent assays (ELISA) of crude tissue extracts. Subcloned plants derived from eight original Ro transformants were inoculated with potyviruses: tobacco etch (TEV), potato virus Y (PVY), and pepper mottle (PeMV). Plants that accumulated detectable levels of the PRV CP showed significant delay in symptom development and the symptoms were attenuated. Similar results were obtained with inoculated R1 plants. We conclude that the expression of the PRV CP-gene imparts protection against infection by a broad spectrum of potyviruses.

• Authors: K Ling, S Namba, C Gonsalves, J L Slightom, D Gonsalves
• Journal: Bio/technology (Nature Publishing Company) (Biotechnology (N Y)) Vol. 9 Issue 8 Pg. 752-8 (Aug 1991) ISSN: 0733-222X [Print] United States
• PMID: 1367635 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• DNA, Viral
• DNA

Topics

• Base Sequence
• Blotting, Southern
• Blotting, Western
• Capsid (biosynthesis, genetics)
• Cloning, Molecular
• DNA (isolation & purification)
• DNA, Viral
• Genes, Viral
• Genetic Vectors
• Molecular Sequence Data
• Plant Diseases
• Plant Viruses (genetics, pathogenicity)
• Plants, Toxic
• Polymerase Chain Reaction
• Tobacco (genetics, immunology, microbiology)
• Transformation, Genetic