HTLV-II-specific antisera raised in rabbits immunized with a synthetic peptide of HTLV-II envelope protein.

Abstract

In order to discriminate HTLV-II from HTLV-I, HTLV-II-specific polyclonal antibodies against a synthetic peptide of HTLV-II envelope sequence were raised in rabbits. We immunized two adult rabbits with a KLH-conjugated synthetic peptide corresponding to the amino acid sequence 171-196 of the HTLV-II envelope sequence, which is a specific region for HTLV-II as evaluated with an ELISA method. The resulting rabbit antisera to the synthetic peptide reacted with gp46 of HTLV-II lysates in Western blot analysis but not with that of HTLV-I. Flow cytometric analysis and immunohistochemical study revealed that these affinity purified antisera recognized some HTLV-II-producing cell lines examined, but not HTLV-I-producing cell lines or other cell lines uninfected by HTLV. These findings indicate that these antisera specifically recognized the envelope glycoprotein (gp46) of HTLV-II and suggest the specificity of this region in the immune response to HTLV-II. Such antisera are useful in distinguishing between HTLV-I and HTLV-II infection and in determining the presence of individual HTLV-II-infected cells both in vivo and in vitro, including non-lymphoid cells. They may also assist in the elucidation of the pathogenesis of HTLV-II.

• Authors: K Hayashi, N Ohara, K Fujiwara, H J Jeon, T R Koirala, K Takahashi, T Akagi, N Tomita, K Miyamoto
• Journal: Virchows Archiv. B, Cell pathology including molecular pathology (Virchows Arch B Cell Pathol Incl Mol Pathol) Vol. 63 Issue 1 Pg. 37-41 ( 1992) ISSN: 0340-6075 [Print] Germany
• PMID: 1362020 (Publication Type: Journal Article)

Chemical References

• Gene Products, env
• HTLV-I Antibodies
• HTLV-II Antibodies
• Retroviridae Proteins, Oncogenic
• env Gene Products, Human Immunodeficiency Virus
• gp46 protein, Human immunodeficiency virus 2

Topics

• Amino Acid Sequence
• Animals
• Gene Products, env (genetics, immunology)
• HTLV-I Antibodies (analysis, immunology)
• HTLV-II Antibodies (analysis, immunology)
• Immunization
• Molecular Sequence Data
• Rabbits
• Retroviridae Proteins, Oncogenic (genetics, immunology)
• env Gene Products, Human Immunodeficiency Virus