Abstract |
Eleven diphenylmethane antihistaminic drugs and their analogues were tested for their detection by capillary gas chromatography (GC) with surface ionization detection ( SID). The GC- SID response was highest for doxylamine, diphenhydramine and orphenadrine and lowest for terodiline, clemastine and pipethanate. The detection limits for drugs with the highest response were 2-5 pg (ca. 6-20 fmol) on-column (100-250 pg/ml of body fluid). The detection limits with GC- SID were 10-100 times higher than those with GC with nitrogen- phosphorus detection. A detailed procedure for the isolation of the antihistaminics from human whole blood and urine by the use of Sep-Pak C18 cartridges, prior to GC- SID, is also presented. The recoveries of the drugs (50 or 500 pmol), which had been added to 1 ml of body fluids, were > 60%. The baselines remained steady as the column temperature was increased and the background was clean, especially for whole blood extracts.
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Authors | H Hattori, S Yamamoto, M Iwata, E Takashima, T Yamada, O Suzuki |
Journal | Journal of chromatography
(J Chromatogr)
Vol. 581
Issue 2
Pg. 213-8
(Oct 23 1992)
Netherlands |
PMID | 1360473
(Publication Type: Journal Article)
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Chemical References |
- Benzhydryl Compounds
- Histamine H1 Antagonists
- Ions
- diphenylmethane
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Topics |
- Benzhydryl Compounds
(analysis, blood, urine)
- Chromatography, Gas
(methods)
- Histamine H1 Antagonists
(analysis, blood, urine)
- Humans
- Ions
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