Close linkage of the olfactory marker protein gene to the mouse deafness mutation shaker-1.

Abstract

One thousand sixty-six progeny have been generated from a backcross segregating for the mouse deafness mutation, shaker-1 (sh-1). One thousand fifty-two mice were analyzed for a protein polymorphism segregating for the distal flanking marker, beta-globin (Hbb), and 13 recombinants between Hbb and sh-1 were identified. One thousand eight mice were analyzed for a restriction fragment length polymorphism segregating for the proximal flanking marker, tyrosinase (c), and 54 recombinants between c and sh-1 were identified, completing a panel of 67 recombinant mice from the backcross in the vicinity of the sh-1 mutation. This panel allows the identification of markers closely linked to the sh-1 mutation that may act as start points for a chromosomal walk to the gene. One such marker, the olfactory marker protein gene (Omp), is recombinant with sh-1 in only one mouse from the recombinant panel. Thus, the Omp gene lies 0.1 cM from sh-1, on average, a distance of 200 kb. Haplotype analysis indicates that Omp lies proximal to sh-1.

• Authors: K A Brown, M J Sutcliffe, K P Steel, S D Brown
• Journal: Genomics (Genomics) Vol. 13 Issue 1 Pg. 189-93 (May 1992) ISSN: 0888-7543 [Print] United States
• PMID: 1349573 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Nerve Tissue Proteins
• Olfactory Marker Protein
• Omp protein, mouse
• Globins
• Monophenol Monooxygenase

Topics

• Animals
• Base Sequence
• Blotting, Southern
• Crosses, Genetic
• Deafness (genetics)
• Female
• Genetic Linkage (genetics)
• Globins (genetics)
• Male
• Mice
• Mice, Inbred Strains
• Molecular Sequence Data
• Monophenol Monooxygenase (genetics)
• Mutation (genetics)
• Nerve Tissue Proteins (genetics)
• Olfactory Marker Protein
• Polymerase Chain Reaction
• Polymorphism, Restriction Fragment Length
• Restriction Mapping