Abstract |
We have identified a 10-kDa stress-inducible mitochondrial protein. The protein is synthesized at elevated rates in cultured rat hepatoma cells challenged with heat shock or amino acid analogues and, therefore, designated heat shock protein 10 (Hsp10). Hsp10 was purified to homogeneity from rat liver and found to exhibit a native molecular mass of 65 kDa, as opposed to a monomeric molecular mass of 10,813.4 +/- 0.41 Da. The amino acid sequence of rat Hsp10 disclosed extensive sequence similarity with bacterial chaperonin (Cpn) 10. Rat Hsp10 and Escherichia coli Cpn60 were used to reconstitute functional trimeric rat ornithine transcarbamoylase from a chemically denatured state with high efficiency. This process depended completely upon rat Hsp10 and was abolished in the presence of a nonhydrolyzable ATP analogue. We conclude that Hsp10 is a eukaryotic Cpn10 homologue and, therefore, together with Cpn60 essential for mitochondrial protein biogenesis. The Cpn-mediated protein-folding apparatus, thus, exhibits a high degree of conservation between prokaryotes and mitochondria of higher eukaryotes.
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Authors | D J Hartman, N J Hoogenraad, R Condron, P B Høj |
Journal | Proceedings of the National Academy of Sciences of the United States of America
(Proc Natl Acad Sci U S A)
Vol. 89
Issue 8
Pg. 3394-8
(Apr 15 1992)
ISSN: 0027-8424 [Print] United States |
PMID | 1348860
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Bacterial Proteins
- Chaperonin 10
- Heat-Shock Proteins
- Macromolecular Substances
- Methionine
- Ornithine Carbamoyltransferase
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Topics |
- Amino Acid Sequence
- Animals
- Bacteria
(genetics)
- Bacterial Proteins
(genetics)
- Chaperonin 10
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
(genetics, metabolism)
- Heat-Shock Proteins
(biosynthesis, genetics, isolation & purification)
- Liver Neoplasms, Experimental
(metabolism)
- Macromolecular Substances
- Methionine
(metabolism)
- Mitochondria
(metabolism)
- Mitochondria, Liver
(metabolism)
- Molecular Sequence Data
- Molecular Weight
- Ornithine Carbamoyltransferase
(chemistry, metabolism)
- Protein Conformation
- Protein Denaturation
- Rats
- Sequence Homology, Nucleic Acid
- Tumor Cells, Cultured
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