Gamma-interferon-induced nerve growth factor receptors in colorectal carcinoma cell lines.

Abstract

Gamma-interferon (gamma IFN) was found to induce expression of the 150,000 M(r) cell surface and the 35,000 M(r) chromatin receptors for nerve growth factor (NGF) in the SW1116 colorectal carcinoma cell line that does not express NGF receptors. In the SW707 colorectal carcinoma cell line that expresses a low level of NGF receptors, gamma IFN stimulated expression of the cell surface and the nuclear receptors. Induction of NGF receptors in SW1116 cells resulted in internalization and nuclear translocation of 125I-NGF. When NGF bound to the chromatin, ribosomal RNA synthesis was inhibited. Two-dimensional gel electrophoresis of [35S]methionine-labeled chromatin proteins indicated significant changes in chromatin protein composition in cells treated and not-treated with gamma IFN. gamma IFN effectively stimulated the expression of NGF receptors in two colorectal carcinoma cell lines, but inhibited the expression in melanoma and breast carcinoma cells. It is suggested that gamma IFN, by modulating the expression of NGF receptors may affect the NGF-dependent growth of some tumor cell lines.

• Authors: E Rakowicz-Szulczynska, J Mozdzanowski, T Lundberg, W Kaczmarski, D Speicher
• Journal: Growth factors (Chur, Switzerland) (Growth Factors) Vol. 6 Issue 4 Pg. 337-46 ( 1992) ISSN: 0897-7194 [Print] England
• PMID: 1340211 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Chromatin
• Nerve Growth Factors
• Nuclear Proteins
• RNA, Neoplasm
• Receptors, Nerve Growth Factor
• Sulfur Radioisotopes
• Interferon-gamma

Topics

• Autoradiography
• Cell Line
• Cell Membrane (metabolism)
• Cell Nucleus (metabolism)
• Chromatin (metabolism)
• Colorectal Neoplasms (metabolism)
• Electrophoresis, Gel, Two-Dimensional
• Electrophoresis, Polyacrylamide Gel
• Gene Expression (drug effects)
• Humans
• Interferon-gamma (pharmacology)
• Melanoma
• Nerve Growth Factors (metabolism)
• Nuclear Proteins (biosynthesis, isolation & purification)
• RNA, Neoplasm (biosynthesis)
• Receptors, Nerve Growth Factor (biosynthesis, drug effects)
• Sulfur Radioisotopes
• Transcription, Genetic (drug effects)
• Tumor Cells, Cultured