[Titration of SH-groups of histone H3 in a DNP preparation of normal and neoplastic animal cells with a mercury-containing spin marker and with a DTNB preparation].

Abstract

DNP samples isolated from the cells of calf thymus and Ehrlich ascite carcinoma of mice were examined. SH-groups of histone H3 of chromatin from these cells were titrated with mercury-containing spin label and with DTNB under joint action of different salt and sarcosyl concentrations on DNP. The results revealed differences in accessibility and titration of histone H3 SH-groups in DNP of normal and tumor cells with DTNB, as well as in molecular dynamics of the mercury-containing spin label introduced to these SH-groups.

• Authors: E M Mil', S S Voronina, V I Biniukov
• Journal: Biofizika (Biofizika) 1992 Sep-Oct Vol. 37 Issue 5 Pg. 868-73 ISSN: 0006-3029 [Print] Russia (Federation)
• Vernacular Title: Titrovanie SH-grupp gistona N3 v DNP normal'nykh i opukholevykh kletok zhivotnykh rtut'soderzhashcheĭ spinovoĭ metkoĭ i preparatom DTNB.
• PMID: 1335288 (Publication Type: English Abstract, Journal Article)

Chemical References

• Deoxyribonucleoproteins
• Histones
• Spin Labels
• Sulfhydryl Compounds
• Dithionitrobenzoic Acid
• Mercury

Topics

• Animals
• Cattle
• Cells, Cultured
• Deoxyribonucleoproteins (chemistry)
• Dithionitrobenzoic Acid (chemistry)
• Electron Spin Resonance Spectroscopy
• Histones (analysis)
• Mercury (chemistry)
• Mice
• Spin Labels
• Sulfhydryl Compounds (chemistry)
• Tumor Cells, Cultured