Abstract |
We have investigated the direct effect of dimethyl prostaglandin A1 ( dmPGA1) on the replication of herpes simplex virus (HSV) and human immunodeficiency virus type 1 (HIV-1). dmPGA1 significantly inhibited viral replication in both HSV and HIV infection systems at concentrations of dmPGA1 that did not adversely alter cellular DNA synthesis. The 50% inhibitory concentration (ID50) for several HSV type 1 (HSV-1) strains ranged from 3.8 to 5.6 micrograms/ml for Vero cells and from 4.6 to 7.3 micrograms/ml for human foreskin fibroblasts. The ID50s for two HSV-2 strains varied from 3.8 to 4.5 micrograms/ml for Vero cells; the ID50 was 5.7 micrograms/ml for human foreskin fibroblasts. We found that closely related prostaglandins did not have the same effect on the replication of HSV; dmPGE2 and dmPGA2 caused up to a 60% increase in HSV replication compared with that in untreated virus-infected cells. HIV-1 replication in acutely infected T cells (VB line) and chronically infected macrophages was assessed by quantitative decreases in p24 concentration. The effective ID50s were 2.5 micrograms/ml for VB cells acutely infected with HIV-1 and 5.2 micrograms/m for chronically infected macrophages. dmPGA1 has an unusual broad-spectrum antiviral activity against both HSV and HIV-1 in vitro and offers a new class of potential therapeutic agents for in vivo use.
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Authors | M Hughes-Fulford, M S McGrath, D Hanks, S Erickson, L Pulliam |
Journal | Antimicrobial agents and chemotherapy
(Antimicrob Agents Chemother)
Vol. 36
Issue 10
Pg. 2253-8
(Oct 1992)
ISSN: 0066-4804 [Print] United States |
PMID | 1332592
(Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Antiviral Agents
- Prostaglandins A, Synthetic
- 16,16-dimethylprostaglandin A1
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Topics |
- Animals
- Antiviral Agents
(pharmacology)
- Cell Line
- Cells, Cultured
- Chlorocebus aethiops
- Fibroblasts
(drug effects, microbiology)
- HIV
(drug effects)
- Humans
- Macrophages
(drug effects, microbiology)
- Prostaglandins A, Synthetic
(pharmacology)
- Simplexvirus
(drug effects)
- Vero Cells
(drug effects)
- Virus Replication
(drug effects)
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