Abstract |
Human replication protein A (RP-A) is a three-subunit protein that is required for simian virus 40 (SV40) replication in vitro. The trypanosome homologue of RP-A has been purified from Crithidia fasciculata. It is a 1:1:1 complex of three polypeptides of 51, 28, and 14 kDa, binds single-stranded DNA via the large subunit, and is localized within the nucleus. C. fasciculata RP-A substitutes for human RP-A in the large tumor antigen-dependent unwinding of the SV40 origin of replication and stimulates both DNA synthesis and DNA priming by human DNA polymerase alpha/primase, but it does not support efficient SV40 DNA replication in vitro. This extraordinary conservation of structure and function between human and trypanosome RP-A suggests that the mechanism of DNA replication, at both the initiation and the elongation level, is conserved in organisms that diverged from the main eukaryotic lineage very early in evolution.
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Authors | G W Brown, T E Melendy, D S Ray |
Journal | Proceedings of the National Academy of Sciences of the United States of America
(Proc Natl Acad Sci U S A)
Vol. 89
Issue 21
Pg. 10227-31
(Nov 01 1992)
ISSN: 0027-8424 [Print] United States |
PMID | 1332038
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- DNA-Binding Proteins
- Macromolecular Substances
- RPA1 protein, human
- Replication Protein A
- DNA Polymerase II
- DNA-Directed DNA Polymerase
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Topics |
- Animals
- Chromatography, Affinity
- Crithidia fasciculata
(genetics, metabolism)
- DNA Polymerase II
(metabolism)
- DNA Replication
- DNA-Binding Proteins
(isolation & purification, metabolism)
- DNA-Directed DNA Polymerase
(metabolism)
- Humans
- Macromolecular Substances
- Replication Protein A
- Simian virus 40
(genetics)
- Templates, Genetic
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