Abstract |
Previous studies have described an augmentation of avian leukosis virus (ALV)-induced lymphoid leukosis in chickens that were coinfected with a serotype 2 Marek's disease virus (MDV) strain, SB-1. As a first step toward understanding the mechanism of this augmentation, we have analyzed the tropism of the MDV for the ALV-transformed B cell. After hatching, chickens were coinfected with ALV and a nonpathogenic strain of MDV, SB-1. Seventy primary and metastatic ALV-induced lymphomas that developed in chickens between 14 and 20 weeks of age were found, with only one exception, to carry SB-1 DNA. The MDV genome was maintained in cell lines derived from the tumors. However, MDV DNA could not be detected in nontransformed bursal B cells from chickens carrying ALV lymphomas. Moreover, during and after the lytic phase of MDV infection, SB-1 DNA was near or below the level of detection in bursal cells, suggesting that MDV most likely infects only a small subpopulation of bursal cells. By contrast, ALV-transformed B cells from MDV-free chickens could be persistently infected with MDV in vitro. These findings indicate that ALV lymphoma cells, unlike nontransformed bursal B cells, are susceptible to persistent MDV infection and can serve as a reservoir of MDV that can potentially influence the physiology of the transformed cell.
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Authors | E Fynan, T M Block, J DuHadaway, W Olson, D L Ewert |
Journal | Journal of virology
(J Virol)
Vol. 66
Issue 10
Pg. 5860-6
(Oct 1992)
ISSN: 0022-538X [Print] United States |
PMID | 1326647
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
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Topics |
- Animals
- Avian Leukosis Virus
(physiology)
- B-Lymphocyte Subsets
- B-Lymphocytes
(cytology, microbiology)
- Blotting, Southern
- Cell Transformation, Neoplastic
- Cell Transformation, Viral
- Chickens
- DNA, Viral
- Herpesvirus 2, Gallid
(isolation & purification, physiology)
- Lymphoma, B-Cell
(genetics, microbiology, pathology)
- Neoplasm Metastasis
- Plasmids
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