Persistence of Marek's disease virus in a subpopulation of B cells that is transformed by avian leukosis virus, but not in normal bursal B cells.

Previous studies have described an augmentation of avian leukosis virus (ALV)-induced lymphoid leukosis in chickens that were coinfected with a serotype 2 Marek's disease virus (MDV) strain, SB-1. As a first step toward understanding the mechanism of this augmentation, we have analyzed the tropism of the MDV for the ALV-transformed B cell. After hatching, chickens were coinfected with ALV and a nonpathogenic strain of MDV, SB-1. Seventy primary and metastatic ALV-induced lymphomas that developed in chickens between 14 and 20 weeks of age were found, with only one exception, to carry SB-1 DNA. The MDV genome was maintained in cell lines derived from the tumors. However, MDV DNA could not be detected in nontransformed bursal B cells from chickens carrying ALV lymphomas. Moreover, during and after the lytic phase of MDV infection, SB-1 DNA was near or below the level of detection in bursal cells, suggesting that MDV most likely infects only a small subpopulation of bursal cells. By contrast, ALV-transformed B cells from MDV-free chickens could be persistently infected with MDV in vitro. These findings indicate that ALV lymphoma cells, unlike nontransformed bursal B cells, are susceptible to persistent MDV infection and can serve as a reservoir of MDV that can potentially influence the physiology of the transformed cell.
AuthorsE Fynan, T M Block, J DuHadaway, W Olson, D L Ewert
JournalJournal of virology (J Virol) Vol. 66 Issue 10 Pg. 5860-6 (Oct 1992) ISSN: 0022-538X [Print] UNITED STATES
PMID1326647 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • DNA, Viral
  • Animals
  • Avian Leukosis Virus (physiology)
  • B-Lymphocyte Subsets
  • B-Lymphocytes (cytology, microbiology)
  • Blotting, Southern
  • Cell Transformation, Neoplastic
  • Cell Transformation, Viral
  • Chickens
  • DNA, Viral
  • Herpesvirus 2, Gallid (isolation & purification, physiology)
  • Lymphoma, B-Cell (genetics, microbiology, pathology)
  • Neoplasm Metastasis
  • Plasmids

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