Abstract |
Production of chromogranin (Cg)A and B derived peptides [ pancreastatin (PST), GAWK, CCB] was studied using human lung carcinoma derived cell lines. PST-like immunoreactivity (LI) was detected in the culture medium in 3 of 6 small cell lung carcinoma (SCLC) cell lines, while GAWK- and CCB-LIs were detected in 5 of 6 and all the 6 SCLC cell lines, respectively. CCB-LI was produced in large amounts in SCLC cell lines as compared to PST- and GAWK-LIs. In non-SCLC cell lines, on the other hand, PST- and GAWK-LIs were not detected. CCB-LI was detected in 1 of 7 non-SCLC cell lines, but not detected in the remainder. PST, GAWK and CCB-LIs, secreted by these cell lines, consisted of several peaks, and these peaks were different among cell lines. This suggests that processing of CgA and B is different in the cell lines. Production of CgA and B derived peptides seems to be a characteristic feature of SCLC, and among them, CCB LI may be a useful marker for SCLC.
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Authors | H Iguchi, S Bannai, N Takanashi, Y Tsukada |
Journal | European journal of cancer (Oxford, England : 1990)
(Eur J Cancer)
Vol. 28A
Issue 8-9
Pg. 1458-62
( 1992)
ISSN: 0959-8049 [Print] England |
PMID | 1325178
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CHGA protein, human
- Chromogranin A
- Chromogranins
- Culture Media
- Neoplasm Proteins
- Peptide Fragments
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Topics |
- Carcinoma, Small Cell
(metabolism)
- Chromatography, Gel
- Chromogranin A
- Chromogranins
(metabolism)
- Culture Media
- Humans
- Lung Neoplasms
(metabolism)
- Neoplasm Proteins
(biosynthesis)
- Peptide Biosynthesis
- Peptide Fragments
(biosynthesis)
- Tumor Cells, Cultured
(metabolism)
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