Abstract |
The incorporation of 2-deoxy-D-galactose into the oligosaccharide moieties of glycoproteins and the consequences of 2-deoxy-D-galactose treatment on the fucosylation of glycoproteins were investigated in the human hepatoma cell line HepG2. Using different methods, it was shown that treatment of HepG2 cells with 2-deoxy-D-galactose leads to an incorporation of 2-deoxy-D-galactose and a decrease of L- fucose incorporation into the oligosaccharides of glycoproteins. The extent of labeling by L-[3H] fucose was determined by removing L-[3H] fucose from labeled cells with the aid of a purified alpha 1,2-fucosidase from Aspergillus niger. Using this method, it was shown that 2-deoxy-D-galactose markedly inhibits alpha 1,2-fucosylation. Measurement of the amount of 2-deoxy-D-galactose incorporated, however, showed that replacement of D-galactose by 2-deoxy-D-galactose does not entirely account for the decrease in alpha 1,2-fucosylation. In addition, a hitherto unreported compensatory increase of alpha 1,3/alpha 1,4-fucosylation was found to occur when alpha-1,2-fucosylation was inhibited by treatment with 2-deoxy-D-galactose.
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Authors | C C Geilen, C Kannicht, B Orthen, C Heidrich, C Paul, D Grunow, R Nuck, W Reutter |
Journal | Archives of biochemistry and biophysics
(Arch Biochem Biophys)
Vol. 296
Issue 1
Pg. 108-14
(Jul 1992)
ISSN: 0003-9861 [Print] United States |
PMID | 1318686
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Carbon Radioisotopes
- Membrane Glycoproteins
- Polysaccharides
- Fucose
- 2-deoxy-lyxo-hexose
- Adenosine Triphosphate
- Galactose
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Topics |
- Adenosine Triphosphate
(metabolism)
- Ascites
(metabolism)
- Carbon Radioisotopes
- Carcinoma, Hepatocellular
- Cell Line
- Cell Membrane
(metabolism)
- Chromatography, Affinity
- Chromatography, Gas
- Chromatography, High Pressure Liquid
- Fucose
(metabolism)
- Galactose
(analogs & derivatives, metabolism)
- Humans
- Kinetics
- Liver Neoplasms
- Membrane Glycoproteins
(biosynthesis, isolation & purification)
- Polysaccharides
(biosynthesis, isolation & purification)
- Radioisotope Dilution Technique
- Tumor Cells, Cultured
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