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Incorporation of the hexose analogue 2-deoxy-D-galactose into membrane glycoproteins in HepG2 cells.

Abstract
The incorporation of 2-deoxy-D-galactose into the oligosaccharide moieties of glycoproteins and the consequences of 2-deoxy-D-galactose treatment on the fucosylation of glycoproteins were investigated in the human hepatoma cell line HepG2. Using different methods, it was shown that treatment of HepG2 cells with 2-deoxy-D-galactose leads to an incorporation of 2-deoxy-D-galactose and a decrease of L-fucose incorporation into the oligosaccharides of glycoproteins. The extent of labeling by L-[3H]fucose was determined by removing L-[3H]fucose from labeled cells with the aid of a purified alpha 1,2-fucosidase from Aspergillus niger. Using this method, it was shown that 2-deoxy-D-galactose markedly inhibits alpha 1,2-fucosylation. Measurement of the amount of 2-deoxy-D-galactose incorporated, however, showed that replacement of D-galactose by 2-deoxy-D-galactose does not entirely account for the decrease in alpha 1,2-fucosylation. In addition, a hitherto unreported compensatory increase of alpha 1,3/alpha 1,4-fucosylation was found to occur when alpha-1,2-fucosylation was inhibited by treatment with 2-deoxy-D-galactose.
AuthorsC C Geilen, C Kannicht, B Orthen, C Heidrich, C Paul, D Grunow, R Nuck, W Reutter
JournalArchives of biochemistry and biophysics (Arch Biochem Biophys) Vol. 296 Issue 1 Pg. 108-14 (Jul 1992) ISSN: 0003-9861 [Print] United States
PMID1318686 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Carbon Radioisotopes
  • Membrane Glycoproteins
  • Polysaccharides
  • Fucose
  • 2-deoxy-lyxo-hexose
  • Adenosine Triphosphate
  • Galactose
Topics
  • Adenosine Triphosphate (metabolism)
  • Ascites (metabolism)
  • Carbon Radioisotopes
  • Carcinoma, Hepatocellular
  • Cell Line
  • Cell Membrane (metabolism)
  • Chromatography, Affinity
  • Chromatography, Gas
  • Chromatography, High Pressure Liquid
  • Fucose (metabolism)
  • Galactose (analogs & derivatives, metabolism)
  • Humans
  • Kinetics
  • Liver Neoplasms
  • Membrane Glycoproteins (biosynthesis, isolation & purification)
  • Polysaccharides (biosynthesis, isolation & purification)
  • Radioisotope Dilution Technique
  • Tumor Cells, Cultured

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