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Isolation of experimental anti-AIDS glycerophospholipids by micro-preparative reversed-phase high-performance liquid chromatography.

Abstract
The experimental anti-AIDS glycerophosphatidic acid: nucleoside (sn-1/sn-2 diacylglycerol:dideoxynucleotide) drugs 3'-azido-3'-deoxythymidine monophosphate diglyceride (AZT-MP-DG) and 2',3'-dideoxycytidine monophosphate diglyceride (ddC-MP-DG) were isolated and purified by reversed-phase high-performance liquid chromatography (HPLC). The chromatographic separation was based on the glycerophospholipid moiety of the drugs and detection of the nucleoside component. The separations were optimized on method development columns packed with the stationary phase to be used in the micro-preparative column and monitored by a UV detector. Fractions were collected and analyzed for purity by analytical-scale HPLC and by thin-layer chromatography (TLC). The purity of the recovered drugs based on UV and light-scattering detection and on TLC was greater than 99%. The purified compounds were isolated for studies on structure confirmation, physical, biophysical and formulation properties and anti-HIV efficacy in culture.
AuthorsJ V Amari, P R Brown, P E Pivarnik, R K Sehgal, J G Turcotte
JournalJournal of chromatography (J Chromatogr) Vol. 590 Issue 1 Pg. 153-61 (Jan 24 1992) Netherlands
PMID1318318 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Dideoxynucleotides
  • Phosphatidic Acids
  • 3'-azido-3'-deoxythymidine monophosphate diglyceride
  • 2',3'-dideoxycytidine monophosphate diglyceride
  • Zidovudine
  • Zalcitabine
Topics
  • Acquired Immunodeficiency Syndrome (drug therapy)
  • Chromatography, High Pressure Liquid
  • Chromatography, Thin Layer
  • Dideoxynucleotides
  • Phosphatidic Acids (isolation & purification, therapeutic use)
  • Spectrophotometry, Ultraviolet
  • Zalcitabine (analogs & derivatives, isolation & purification, therapeutic use)
  • Zidovudine (analogs & derivatives, isolation & purification, therapeutic use)

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