Abstract |
A HT 29 cell line derived from human colonic carcinoma was shown to express the intrinsic factor receptor, with about 5000 binding sites per cell and an association constant of 20 x 10(9) 1/mol at pH 7.4 and 4 degrees C. The number of binding sites increased dramatically between 7 and 10 days of culture time. Endocytosis of the intrinsic factor-cobalamin-receptor complex was inhibited by two ways: at 4 degrees C and at 37 degrees C by incubating the cells with vinblastine, monensin and chloroquine. The plasma membrane receptor was cross-linked to [57Co] cobalamin- intrinsic factor and solubilized with Triton X-100. The cross-linked complex had a relative molecular mass of 330 kDa in native PAGE.
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Authors | J L Guéant, C Masson, H Schohn, M Girr, M Saunier, J P Nicolas |
Journal | FEBS letters
(FEBS Lett)
Vol. 297
Issue 3
Pg. 229-32
(Feb 10 1992)
ISSN: 0014-5793 [Print] England |
PMID | 1312012
(Publication Type: Journal Article)
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Chemical References |
- Receptors, Cell Surface
- Chloroquine
- Intrinsic Factor
- Vitamin B 12
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Topics |
- Chloroquine
(pharmacology)
- Chromatography, Gel
- Colonic Neoplasms
- Electrophoresis, Polyacrylamide Gel
- Endocytosis
- Humans
- Intrinsic Factor
(metabolism)
- Kinetics
- Receptors, Cell Surface
(metabolism)
- Tumor Cells, Cultured
- Vitamin B 12
(metabolism)
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