Evaluation of a protease activation mutant of Sendai virus as a potent live vaccine.

Abstract

A protease activation mutant of Sendai virus, TR-5, was investigated as a candidate for a live vaccine. Vaccination with TR-5 which had been activated by chymotrypsin beforehand (active TR-5) elicited protective immunity against otherwise lethal challenge infection with wild-type Sendai virus in DBA/2, C3H and ICR strains of mice. Less of the active TR-5 was required to confer protection on mice compared with an ordinary ether-inactivated Sendai virus vaccine (split vaccine). The protective immunity elicited by TR-5 lasted longer and the booster effect was more prominent compared to the split vaccine. No seroconversion was observed with contact mice when housed in a cage with mice vaccinated with the active TR-5. The overall results show that the active TR-5 is an effective and safe live vaccine of Sendai virus in mice.

• Authors: M Maru, M Haraguchi, K Sato, H Hotta, M Homma
• Journal: Veterinary microbiology (Vet Microbiol) Vol. 30 Issue 1 Pg. 1-12 (Jan 1992) ISSN: 0378-1135 [Print] Netherlands
• PMID: 1311131 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Aerosols
• Antibodies, Viral
• Vaccines, Attenuated
• Viral Vaccines

Topics

• Administration, Inhalation
• Aerosols
• Animals
• Antibodies, Viral (biosynthesis)
• Female
• Hemagglutination Inhibition Tests
• Immunization, Secondary (veterinary)
• Mice
• Mice, Inbred C3H
• Mice, Inbred DBA
• Mice, Inbred ICR
• Mice, Inbred Strains
• Mutation
• Parainfluenza Virus 1, Human (genetics, immunology)
• Paramyxoviridae Infections (prevention & control, veterinary)
• Rodent Diseases (prevention & control)
• Vaccination (veterinary)
• Vaccines, Attenuated (administration & dosage)
• Viral Vaccines (administration & dosage)