Abstract |
Our previous studies showed that phenethyl isothiocyanate ( PEITC), a cruciferous vegetable constituent, inhibited the lung tumorigenesis induced by a potent tobacco-specific carcinogenic nitrosamine in animals. These results implicate dietary PEITC as a risk-reducing factor of lung cancers induced by smoking. To define the effect of dietary PEITC on human cancers, a method of measuring its uptake is needed. Since watercress is rich in gluconasturtiin, a glucosinolate precursor of PEITC, it was chosen to be the source of PEITC. Four individuals were asked to eat watercress as part of a breakfast meal, and 24-h urine samples were collected. A urinary metabolite was found, and its identity was confirmed as the N-acetylcysteine conjugate of PEITC by comparison with the synthetic standard using nuclear magnetic resonance and mass spectrometry. A dose-dependent excretion of this conjugate was observed. These results clearly showed that PEITC was released in the human body upon ingestion of watercress and suggest that the N-acetylcysteine conjugate of PEITC may be a useful marker for quantitating human exposure to this anticarcinogen as a tool for epidemiological investigations.
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Authors | F L Chung, M A Morse, K I Eklind, J Lewis |
Journal | Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
(Cancer Epidemiol Biomarkers Prev)
1992 Jul-Aug
Vol. 1
Issue 5
Pg. 383-8
ISSN: 1055-9965 [Print] United States |
PMID | 1305471
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Anticarcinogenic Agents
- Glucosinolates
- Isothiocyanates
- Thiocyanates
- phenethyl isothiocyanate
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Topics |
- Animals
- Anticarcinogenic Agents
(metabolism, pharmacokinetics, urine)
- Chromatography, High Pressure Liquid
(methods, standards)
- Dose-Response Relationship, Drug
- Eating
- Evaluation Studies as Topic
- Female
- Glucosinolates
(chemistry, metabolism)
- Humans
- Isothiocyanates
- Lung Neoplasms
(diet therapy, prevention & control)
- Magnetic Resonance Spectroscopy
- Male
- Mass Spectrometry
- Metabolic Clearance Rate
- Mice
- Thiocyanates
(metabolism, pharmacokinetics, urine)
- Vegetables
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