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Gene and protein expression profiling during differentiation of neuroblastoma cells triggered by 13-cis retinoic acid.

AbstractPURPOSE:
The precise changes in RNA and protein expression that accompany neuroblastoma differentiation remain unknown. The authors used microarray technologies to screen molecules associated with the differentiation of neuroblastoma (NB) cells induced by 13-cis retinoic acid.
METHODS:
The authors quantified the expression of 2,061 RNA transcripts related to oncogenesis and of 380 proteins expressed in SK-N-SH and CHP-134 NB cell lines in the presence or absence of 13-cis retinoic acid.
RESULTS:
Hierarchical clustering captured gene expression altered during neuroblastoma differentiation induced by 13-cis retinoic acid. Several genes were further abstracted based on P values below 0.0017 or protein chips observed in both NB cell lines. The altered expressions of gene products revealed by both DNA and protein chips were in agreement. The expressions of N-myc, cyclin D3, and Wnt10B were downregulated, whereas those of retinoblastoma (RB) and related genes (p107, RB2/p130, p300/CBP, E2F-1, DP-1) as well as others were upregulated.
CONCLUSIONS:
These results suggest that microarray technology can screen for genes that are important in neuroblastoma differentiation.
AuthorsYuki Yuza, Miyuki Agawa, Masaharu Matsuzaki, Hisashi Yamada, Mitsuyoshi Urashima
JournalJournal of pediatric hematology/oncology (J Pediatr Hematol Oncol) Vol. 25 Issue 9 Pg. 715-20 (Sep 2003) ISSN: 1077-4114 [Print] United States
PMID12972807 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antineoplastic Agents
  • Neoplasm Proteins
  • RNA, Messenger
  • RNA, Neoplasm
  • Isotretinoin
Topics
  • Antineoplastic Agents (pharmacology)
  • Cell Differentiation (drug effects, genetics)
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic (drug effects)
  • Humans
  • Isotretinoin (pharmacology)
  • Neoplasm Proteins (biosynthesis, genetics)
  • Neuroblastoma (genetics, metabolism, pathology)
  • Oligonucleotide Array Sequence Analysis
  • Protein Array Analysis
  • RNA, Messenger (biosynthesis)
  • RNA, Neoplasm (biosynthesis)
  • Tumor Cells, Cultured (drug effects, metabolism)

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