| Abstract | OBJECTIVE: To study the effect of weight loss in response to a lifestyle modification program on the circulating levels of adipose tissue derived cytokines (adipokines) in obese individuals with insulin resistance. RESEARCH METHODS AND PROCEDURES: Twenty-four insulin-resistant obese subjects with varying degrees of glucose tolerance completed a 6-month program consisting of combined hypocaloric diet and moderate physical activity. Adipokines [leptin, adiponectin, resistin, tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6)] and highly sensitive C-reactive protein were measured before and after the intervention. Insulin sensitivity index was evaluated by the frequently sampled intravenous glucose tolerance test. RESULTS: Participants had a 6.9 +/- 0.1 kg average weight loss, with a significant improvement in sensitivity index and reduction in plasma leptin (27.8 +/- 3 vs. 23.6 +/- 3 ng/mL, p = 0.01) and IL-6 (2.75 +/- 1.51 vs. 2.3 +/- 0.91 pg/mL, p = 0.012). TNF-alpha levels tended to decrease (2.3 +/- 0.2 vs. 1.9 +/- 0.1 pg/mL, p = 0.059). Adiponectin increased significantly only among diabetic subjects. The reductions in leptin were correlated with the decreases in BMI (r = 0.464, p < 0.05) and with changes in highly sensitive C-reactive protein (r = 0.466, p < 0.05). DISCUSSION: Weight reduction in obese individuals with insulin resistance was associated with a significant decrease in leptin and IL-6 and a tendency toward a decrease in circulating TNF-alpha, whereas adiponectin was increased only in diabetic subjects. Further studies are needed to elucidate the relationship between changes of adipokines and the health benefits of weight loss. |
| Authors | Lais U Monzillo, Osama Hamdy, Edward S Horton, Sarah Ledbury, Cathy Mullooly, Catherine Jarema, Satoko Porter, Kerry Ovalle, Amr Moussa, Christos S Mantzoros
(Affiliation: Clinical Research Center, Joslin Diabetes Center, and Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA.)
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| Journal | Obesity research
(Obes Res)
Vol. 11
Issue 9
Pg. 1048-54
(Sep 2003)
ISSN: 1071-7323 United States |
| PMID | 12972674
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
|
| Chemical References |
- Adiponectin
- Blood Glucose
- Cytokines
- Hormones, Ectopic
- Intercellular Signaling Peptides and Proteins
- Interleukin-6
- Leptin
- Proteins
- RETN protein, human
- Resistin
- Tumor Necrosis Factor-alpha
- C-Reactive Protein
|
| Topics |
- Adiponectin
- Blood Glucose
(analysis)
- Body Mass Index
- C-Reactive Protein
(analysis)
- Cytokines
(blood)
- Diabetes Mellitus, Type 2
(blood)
- Diet, Reducing
- Exercise
(physiology)
- Glucose Tolerance Test
- Hormones, Ectopic
(blood)
- Humans
- Insulin Resistance
- Intercellular Signaling Peptides and Proteins
- Interleukin-6
(blood)
- Leptin
(blood)
- Life Style
- Middle Aged
- Obesity
(blood, physiopathology, therapy)
- Proteins
(analysis)
- Resistin
- Tumor Necrosis Factor-alpha
(analysis)
|
