Abstract |
Modifying potential of annatto extract ( norbixin) on liver carcinogenesis was investigated in male F344/DuCrj rats initially treated with N-nitrosodiethylamine (DEN). Two weeks after a single dose of DEN (200 mg/kg, intraperitoneally), rats were given annatto extract at dietary levels of 0, 0.03, 0.1 and 0.3%, or phenobarbital sodium at 0.05% as a positive control for 6 weeks. All animals were subjected to partial hepatectomy at week 3, and were killed at week 8. There were no deaths related to annatto extract ingestion, and the treatment had no effects on body weights, or food and water consumption. Statistically significant increases of absolute and relative liver weights were apparent in the 0.1 and 0.3% groups. However, annatto extract did not significantly increase the quantitative values for glutathione S-transferase placental form positive liver cell foci observed after DEN initiation, in clear contrast to the positive control case. The results thus demonstrate that annatto extract at a dietary level of 0.3% (200 mg/kg/day) lacks modifying potential for liver carcinogenesis in our medium-term bioassay system.
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Authors | Akihiro Hagiwara, Norio Imai, Yuko Doi, Kyoko Nabae, Takeshi Hirota, Hiroko Yoshino, Mayumi Kawabe, Yoko Tsushima, Hiromitsu Aoki, Kazuo Yasuhara, Takatoshi Koda, Mikio Nakamura, Tomoyuki Shirai |
Journal | Cancer letters
(Cancer Lett)
Vol. 199
Issue 1
Pg. 9-17
(Sep 10 2003)
ISSN: 0304-3835 [Print] Ireland |
PMID | 12963118
(Publication Type: Journal Article)
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Chemical References |
- Carcinogens
- Food Coloring Agents
- Carotenoids
- norbixin
- Phenobarbital
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Topics |
- Animals
- Body Weight
(drug effects)
- Carcinogenicity Tests
- Carcinogens
(toxicity)
- Carotenoids
(toxicity)
- Disease Models, Animal
- Food Coloring Agents
- Liver
(anatomy & histology, drug effects)
- Liver Neoplasms
(chemically induced, pathology)
- Male
- Organ Size
(drug effects)
- Phenobarbital
(toxicity)
- Rats
- Rats, Inbred F344
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