The tumor promoting effect of lime-piper betel quid in JB6 cells.

Betel quid chewing is a general behavior in Taiwan, India, southeastern Asian and South Africa. In this study, microculture tetrazolium test (MTT) showed that the extract of lime-piper betel quid (LPB) (1.0-20 mg/ml) was toxic to JB6 cells. Cells exposed of LPB (0.1, 0.5, 1.0 mg/ml) for 7 days resulted in changes in cytomorphology with characteristics of carcinogenesis. With a long-term treatment (approximately 30 days) of low doses of LPB (1, 5, 10 microg/ml), the production of H2O2 and the activity of myeloperoxidase (MPO) and ornithine decarboxylase (ODC) were increased in JB6 cells. Cell cycle analysis showed a decrease in the G1 phase and an accumulation in the S phase 48 h after LPB treatment. When treating with 0.5 mg/ml LPB for 15 days as a promoter, type III foci were formed in the JB6 culture. These results demonstrated the tumor promotional effect of LPB in JB6 cells.
AuthorsMing-Hsun Lin, Fen-Pi Chou, Hui-Pei Huang, Jeng-Dong Hsu, Ming-Yung Chou, Chau-Jong Wang
JournalFood and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 41 Issue 11 Pg. 1463-71 (Nov 2003) ISSN: 0278-6915 [Print] England
PMID12962998 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Calcium Compounds
  • Carcinogens
  • Oxides
  • Plant Extracts
  • Tetrazolium Salts
  • Thiazoles
  • lime-piper betel quid
  • thiazolyl blue
  • Hydrogen Peroxide
  • Peroxidase
  • Ornithine Decarboxylase
  • Animals
  • Areca (toxicity)
  • Calcium Compounds (toxicity)
  • Carcinogenicity Tests
  • Carcinogens (toxicity)
  • Cell Cycle (drug effects)
  • Cell Line
  • Cell Transformation, Neoplastic (drug effects)
  • Citrus aurantiifolia (toxicity)
  • G1 Phase (drug effects)
  • Hydrogen Peroxide (chemistry)
  • Mice
  • Ornithine Decarboxylase (metabolism)
  • Oxides (toxicity)
  • Peroxidase (metabolism)
  • Piper (toxicity)
  • Plant Extracts (toxicity)
  • S Phase (drug effects)
  • Tetrazolium Salts
  • Thiazoles

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