Abstract | PURPOSE: EXPERIMENTAL DESIGN: We performed reverse transcription-PCR analysis for CCR7 in 20 esophageal SCC cell lines and immunohistochemical analysis of 96 esophageal SCC samples. We then performed a cell migration assay, F-actin polymerization, and a phagokinetic assay on esophageal SCC cell lines in the presence of CCL21, a ligand of CCR7. RESULTS: CCR7 mRNA was detected in 9 of 20 esophageal SCC cell lines. Immunoreactive CCR7 was found mainly in esophageal cancer cells. High CCR7 expression was significantly correlated with esophageal SCC lymphatic permeation, lymph node metastasis, tumor depth, and tumor-node- metastasis stage and was associated with poor survival. In vitro studies demonstrated that CCL21 significantly increased the cell migration ability of esophageal SCC cell lines, and pseudopodia formation was induced by CCL21 stimulation. Furthermore, CCL21 markedly enhanced the motility of esophageal carcinoma cell lines by the phagokinetic assay. CONCLUSIONS:
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Authors | Yongzeng Ding, Yutaka Shimada, Masato Maeda, Atsushi Kawabe, Junichi Kaganoi, Izumi Komoto, Yosuke Hashimoto, Masayuki Miyake, Hiroki Hashida, Masayuki Imamura |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 9
Issue 9
Pg. 3406-12
(Aug 15 2003)
ISSN: 1078-0432 [Print] United States |
PMID | 12960129
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CCR7 protein, human
- Ligands
- RNA, Messenger
- Receptors, CCR7
- Receptors, Chemokine
- RNA
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Topics |
- Aged
- Carcinoma, Squamous Cell
(pathology)
- Cell Line, Tumor
- Cell Movement
- Chemotaxis
- Dendritic Cells
(metabolism)
- Esophageal Neoplasms
(pathology)
- Female
- Humans
- Immunohistochemistry
- Ligands
- Lymphatic Metastasis
- Male
- Microscopy, Confocal
- Microscopy, Fluorescence
- Middle Aged
- RNA
(metabolism)
- RNA, Messenger
(metabolism)
- Receptors, CCR7
- Receptors, Chemokine
(biosynthesis)
- Reverse Transcriptase Polymerase Chain Reaction
- Time Factors
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