Use of multiplex PCR to identify Staphylococcus aureus adhesins involved in human hematogenous infections.

Abstract

We have developed a multiplex PCR procedure to determine the distribution of nine adhesin genes in Staphylococcus aureus isolates. Only genes encoding bone sialoprotein binding protein and fibronectin binding protein B were significantly associated with hematogenous osteomyelitis/arthritis and native-valve endocarditis, respectively, suggesting their involvement in hematogenous tissue infections.

• Authors: Anne Tristan, Liu Ying, Michele Bes, Jerome Etienne, Francois Vandenesch, Gerard Lina
• Journal: Journal of clinical microbiology (J Clin Microbiol) Vol. 41 Issue 9 Pg. 4465-7 (Sep 2003) ISSN: 0095-1137 [Print] United States
• PMID: 12958296 (Publication Type: Journal Article)

Chemical References

• Adhesins, Bacterial
• Bacterial Proteins
• Carrier Proteins
• Coagulase
• adhesin, Staphylococcus aureus
• fibronectin-binding proteins, bacterial

Topics

• Adhesins, Bacterial (genetics, physiology)
• Arthritis, Infectious (microbiology)
• Bacterial Proteins (genetics)
• Carrier Proteins (genetics)
• Coagulase (genetics)
• Endocarditis, Bacterial (microbiology)
• Humans
• Nose (microbiology)
• Osteomyelitis (microbiology)
• Polymerase Chain Reaction (methods)
• Sensitivity and Specificity