Abstract | BACKGROUND: Premalignant Barrett's oesophagus (BO) and gastric intestinal metaplasia (IM) show phenotypic variability. Incompletely differentiated sulfomucin rich gastric IM (type III) may have increased malignant potential. The types of sulfated oligosaccharide structures present in IM, BO, and colon have not been fully characterised. AIMS: METHODS: Sections containing gastric IM or BO (some associated with dysplasia or adenocarcinoma) were stained by the HID/ alcian blue (AB) method and immunohistochemically (antibody 91.9H) to detect sulfo-Lewis(a). Based on HID/AB staining, IM was subtyped into type I (complete) or types II and III (incomplete). RESULTS: CONCLUSIONS:
Sulfo-Le(a), which is expressed on colonic mucin, is invariably present on sulfomucins in gastric IM and BO. Its presence in incomplete variants of IM and its absence from type I IM emphasises the phenotypic differences between complete and incomplete forms of metaplasia. 91.9H immunostaining is useful in IM subtyping. Characterising the molecular basis of sulfo-Lewis(a) expression may help understand the process of aberrant differentiation.
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Authors | K Bodger, F Campbell, J M Rhodes |
Journal | Journal of clinical pathology
(J Clin Pathol)
Vol. 56
Issue 9
Pg. 703-8
(Sep 2003)
ISSN: 0021-9746 [Print] England |
PMID | 12944557
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Coloring Agents
- Lewis Blood Group Antigens
- Mucins
- Neoplasm Proteins
- Oligosaccharides
- sulfo-Lewis(a)
- sulfomucin
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Topics |
- Adenocarcinoma
(chemistry)
- Barrett Esophagus
(metabolism)
- Colon
(chemistry)
- Coloring Agents
- Esophagus
(chemistry, pathology)
- Humans
- Immunohistochemistry
(methods)
- Intestinal Mucosa
(chemistry)
- Intestinal Neoplasms
(chemistry)
- Intestines
(chemistry, pathology)
- Lewis Blood Group Antigens
- Metaplasia
- Mucins
(analysis)
- Neoplasm Proteins
(analysis)
- Oligosaccharides
(analysis)
- Sensitivity and Specificity
- Stomach Neoplasms
(chemistry)
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