Abstract | PURPOSE: METHODS: The expression of all three members of the SK channel family was quantified by PCR. Their functional activity was investigated by using electrophysiological and calcium-imaging techniques. Lens voltage was monitored by inserting a single electrode into the intact human lens, and changes in intracellular calcium were recorded simultaneously after fura-2 incorporation. RESULTS: CONCLUSIONS: SK channels are an integral part of the G-protein and tyrosine kinase calcium signaling mechanisms in the human lens, and their activation is inhibited by certain anti-psychotic drugs. These findings help explain why a change in channel activity, whether by abnormal gene expression or by drug intervention, can lead to cataract.
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Authors | Jeremy D Rhodes, David J Collison, George Duncan |
Journal | Investigative ophthalmology & visual science
(Invest Ophthalmol Vis Sci)
Vol. 44
Issue 9
Pg. 3927-32
(Sep 2003)
ISSN: 0146-0404 [Print] United States |
PMID | 12939311
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Calcium Channel Agonists
- Potassium Channels
- Potassium Channels, Calcium-Activated
- Small-Conductance Calcium-Activated Potassium Channels
- Trifluoperazine
- Apamin
- Ionomycin
- Protein-Tyrosine Kinases
- GTP-Binding Proteins
- Calcium
- Fura-2
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Topics |
- Aged
- Aged, 80 and over
- Apamin
(pharmacology)
- Calcium
(physiology)
- Calcium Channel Agonists
(pharmacology)
- Calcium Signaling
(physiology)
- Electrophysiology
- Fura-2
- GTP-Binding Proteins
(agonists, metabolism)
- Humans
- Ionomycin
(pharmacology)
- Lens, Crystalline
(drug effects, metabolism)
- Membrane Potentials
(drug effects)
- Middle Aged
- Polymerase Chain Reaction
- Potassium Channels
(genetics, metabolism)
- Potassium Channels, Calcium-Activated
- Protein-Tyrosine Kinases
(metabolism)
- Small-Conductance Calcium-Activated Potassium Channels
- Trifluoperazine
(pharmacology)
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