Predictive value of hypo-osmotic swelling test to identify viable non-motile sperm.

Abstract

AIM:

To determine the predictive value of the hypo-osmotic swelling (HOS) test to identify viable, non-motile sperm.

METHODS:

Semen samples from 20 men with severe asthenozoospermia underwent traditional seminal analysis, eosin-nigrosin (EN) staining and the HOS test. A further EN stain was then performed on a HOS pre-treated aliquot and a total of 2000 further sperm examined.

RESULTS:

The median sperm density was 5.1 million/mL (IQR 4.3 approximately 13.1) and the median motility was 3.0% (IQR 0 approximately 7). Seven samples showed complete asthenozoospermia. Initial EN staining showed 59% viability (range 48 approximately 69) despite the poor standard parameters and 47% (range 33 approximately 61) in the complete asthenozoospermia subgroup. The HOS test showed 49.9% reacted overall (range 40 approximately 59) and 41.7% (range 22 approximately 61) in the complete asthenozoospermia subgroup. The combined HOS/EN stain showed the positive predictive value of the HOS test to identify viable sperm was 84.2 % overall and 79.7% in the complete asthenozoospermia subgroup.

CONCLUSION:

The HOS test can effectively predict sperm viability in patients with severe and complete asthenozoospermia.

• Authors: William M Buckett
• Journal: Asian journal of andrology (Asian J Androl) Vol. 5 Issue 3 Pg. 209-12 (Sep 2003) ISSN: 1008-682X [Print] China
• PMID: 12937803 (Publication Type: Journal Article)

Chemical References

• Aniline Compounds
• Coloring Agents
• Fluorescent Dyes
• nigrosin
• Eosine Yellowish-(YS)

Topics

• Adult
• Aniline Compounds
• Cell Survival (physiology)
• Coloring Agents
• Eosine Yellowish-(YS)
• Fluorescent Dyes
• Humans
• In Vitro Techniques
• Male
• Osmotic Pressure
• Predictive Value of Tests
• Semen (cytology)
• Sperm Motility (physiology)
• Spermatozoa (physiology, ultrastructure)