Correlation of photosensitizer delivery to lipoproteins and efficacy in tumor and arthritis mouse models; comparison of lipid-based and Pluronic P123 formulations.

Abstract

PURPOSE:

The purpose of this study was the use of animal models to demonstrate the importance of drug delivery (verteporfin) to plasma lipoproteins in order to attain efficacy of photodynamic therapy (PDT) in vivo.

METHODS:

Photosensitizers appropriately formulated in various vehicles such as pluronics and lipid-based systems were compared to delivery of the drug in DMSO in two in vivo systems. The first was a tumor model using male DBA/2 mice inoculated intradermally with M1 rhabdomyosarcoma cells and in the second, arthritis in the MRL -lpr mouse strain was enhanced by two intradermal injections of complete Freunds adjunct.

RESULTS:

Those formulations in which the drug was in a monomeric form were better able to transfer drug to lipoproteins, which in turn led to superior PDT in vivo.

CONCLUSIONS:

The ability to introduce drug in monomeric form into the circulation correlates well with efficacy of photosensitizer formulations in mouse arthritis and tumor models.

• Authors: Rubinah K Chowdhary, Isha Sharif, Namrata Chansarkar, David Dolphin, Leslie Ratkay, Sean Delaney, Howard Meadows
• Journal: Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques (J Pharm Pharm Sci) 2003 May-Aug Vol. 6 Issue 2 Pg. 198-204 ISSN: 1482-1826 [Electronic] Canada
• PMID: 12935430 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Lipoproteins
• Photosensitizing Agents

Topics

• Animals
• Chemistry, Pharmaceutical
• Drug Delivery Systems
• Lipoproteins (metabolism)
• Male
• Mice
• Mice, Inbred DBA
• Photochemotherapy
• Photosensitizing Agents (administration & dosage)
• Plasma (metabolism)