Cocaine- and amphetamine-regulated transcript peptide attenuates phenylephrine-induced bradycardia in anesthetized rats.

Abstract	The present study was undertaken to investigate the origin of cocaine- and amphetamine-regulated transcript (CART) peptide immunoreactive (irCART) fibers observed in the nucleus of the solitary tract (NTS) and assess the role of CART peptide on phenylephrine (PE)-induced baroreflex. Immunohistochemical and retrograde tract-tracing studies showed that some of the irCART fibers observed in the NTS may have their cell bodies in the nodose ganglia. In urethane-anesthetized rats, intracisternal or bilateral intra-NTS microinjection of the CART peptide fragment 55-102 (0.1-3 nmol), referred to herein as CARTp, consistently and dose dependently attenuated PE-induced bradycardia. CARTp, in the doses used here, caused no significant changes of resting blood pressure or heart rate. Bilateral intra-NTS injections of CART antibody (1:500) potentiated PE-induced bradycardia. Injections of saline, normal rabbit serum, or concomitant injection of CARTp and CART antiserum into the NTS caused no significant changes of PE-induced baroreflex. The result suggests that endogenously released CARTp from primary afferents or exogenously administered CARTp modulates PE-induced baroreflex.
Authors	Phouangmala Scruggs, Siok L Dun, Nae J Dun
Journal	American journal of physiology. Regulatory, integrative and comparative physiology (Am J Physiol Regul Integr Comp Physiol) Vol. 285 Issue 6 Pg. R1496-503 (Dec 2003) ISSN: 0363-6119 [Print] United States
PMID	12933358 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References	Antibodies Blood Proteins Cardiotonic Agents Nerve Tissue Proteins cocaine- and amphetamine-regulated transcript protein Phenylephrine Sodium Chloride
Topics	Anesthesia Animals Antibodies (pharmacology) Baroreflex (physiology) Blood Proteins (pharmacology) Bradycardia (chemically induced, physiopathology) Cardiotonic Agents Heart Rate (drug effects) Immunohistochemistry Male Nerve Fibers (physiology) Nerve Tissue Proteins (immunology, pharmacology, physiology) Neural Pathways Nodose Ganglion (cytology, physiology) Phenylephrine Rats Rats, Sprague-Dawley Sodium Chloride (pharmacology) Solitary Nucleus (cytology, physiology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!