Effects of PTX1 expression on growth and tumorigenicity of the prostate cancer cell line PC-3.

Abstract

PTX1 is a gene identified by subtractive hybridization on the basis that it is expressed in normal prostate and not in prostate carcinoma. It encodes a nuclear protein that is downregulated in prostate carcinoma. Expression constructs containing PTX1 cDNA in both sense and antisense orientations were transfected into prostate tumor cell line, PC-3 cells. The effects of the expression of PTX1 and antisense PTX1 on PC-3 cells were examined using cell growth, proliferation, soft agar, invasion chamber, senescence-associated beta-galactosidase, and nude mice assays. Cells transfected with PTX1 construct in the sense orientation were growth-arrested. These cells displayed multiple morphological changes consistent with cellular senescence, including the expression of a senescence-associated beta-galactosidase. On the other hand, expression of antisense PTX1 RNA in PC-3 cells resulted in uncontrolled cell growth and increase of invasive potential. In nude mice, cells expressing antisense PTX1 grew sixfold faster than the control. These results suggest that PTX1 may play an important role in the growth and tumorigenicity of PC-3 cells.

• Authors: Xianhong Liu, Ierachmiel Daskal, Simon C M Kwok
• Journal: DNA and cell biology (DNA Cell Biol) Vol. 22 Issue 7 Pg. 469-74 (Jul 2003) ISSN: 1044-5498 [Print] United States
• PMID: 12932305 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Antisense
• DNA, Complementary
• ERGIC2 protein, human
• Nuclear Proteins
• Vesicular Transport Proteins

Topics

• Animals
• Cell Division (genetics)
• Cellular Senescence (genetics)
• DNA, Antisense (genetics)
• DNA, Complementary (genetics)
• Gene Expression
• Genes, Tumor Suppressor
• Humans
• Male
• Mice
• Mice, Nude
• Nuclear Proteins (genetics)
• Prostatic Neoplasms (genetics, pathology)
• Transfection
• Tumor Cells, Cultured
• Vesicular Transport Proteins