Evaluation of Venezuelan Equine Encephalitis (VEE) replicon-based Outer surface protein A (OspA) vaccines in a tick challenge mouse model of Lyme disease.

Abstract	Venezuelan Equine Encephalitis (VEE) virus replicon particles (VRPs) encoding Borrelia burgdorferi Outer surface protein A (OspA) were evaluated for their ability to induce an immune response and provide protection from tick-borne spirochetes. VRPs expressing ospA that accumulated intracellularly (VRP OspA) or that was secreted from host cells (VRP tPA-OspA) were tested. Both VRP OspA and VRP tPA-OspA expressed ospA in immunized mice. Mice vaccinated with VRPs expressing secreted OspA produced significant amounts of anti-OspA antibodies, whereas VRPs expressing intracellular OspA were less immunogenic. The VRP method of delivery induced a Th1 type immune response unlike the recombinant OspA protein in Freund's adjuvant, which induced a mixed (Th1 and Th2) immune response. The VRP tPA-OspA construct induced an immune response that reduced the bacterial load in feeding Ixodes scapularis and blocked transmission to the host. These results indicate that VRPs are capable of providing protection against tick-borne B. burgdorferi, and potentially can be used for developing improved vaccines against Lyme disease.
Authors	Clay L Gipson, Nancy L Davis, Robert E Johnston, Aravinda M de Silva
Journal	Vaccine (Vaccine) Vol. 21 Issue 25-26 Pg. 3875-84 (Sep 08 2003) ISSN: 0264-410X [Print] Netherlands
PMID	12922122 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References	Adjuvants, Immunologic Antigens, Surface Bacterial Outer Membrane Proteins Bacterial Vaccines Immunoglobulin G Lipoproteins Lyme Disease Vaccines OspA protein RNA, Viral
Topics	Adjuvants, Immunologic (pharmacology) Animals Antigens, Surface (genetics, immunology) Bacterial Outer Membrane Proteins (genetics, immunology) Bacterial Vaccines Cells, Cultured Cricetinae Encephalitis Virus, Venezuelan Equine (genetics, immunology) Enzyme-Linked Immunosorbent Assay Immunoglobulin G (biosynthesis) Immunohistochemistry Lipoproteins Lyme Disease (immunology, microbiology, prevention & control) Lyme Disease Vaccines (immunology) Mice Mice, Inbred C3H Plasmids (genetics) RNA, Viral (biosynthesis, genetics) Replicon (genetics, immunology) Ticks (microbiology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!