Honokiol and magnolol induce Ca2+ mobilization in rat cortical neurons and human neuroblastoma SH-SY5Y cells.

Abstract

We examined the intracellular Ca(2+) response in primary cultured rat cortical neurons and human neuroblastoma SH-SY5Y cells by Fluo 3 fluorescence imaging analysis. In these two kinds of neuronal cells, honokiol and magnolol increased cytoplasmic free Ca(2+) with a characteristic lag phase. The cytoplasmic free Ca(2+) increase was independent of extracellular Ca(2+), but dependent on activation of phospholipase C and inositol 1,4,5-triphosphate (IP(3)) receptors. These results suggest that honokiol and magnolol increase cytoplasmic free Ca(2+) through a phospholipase C-mediated pathway, and that the release of Ca(2+) from intracellular stores mainly contributes to the increase in cytoplasmic free Ca(2+). Thus, honokiol and magnolol may be involved in a new activation mechanism closely associated with intracellular Ca(2+) mobilization.

• Authors: Haifeng Zhai, Kousuke Nakade, Yasuhide Mitsumoto, Yoshiyasu Fukuyama
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 474 Issue 2-3 Pg. 199-204 (Aug 08 2003) ISSN: 0014-2999 [Print] Netherlands
• PMID: 12921862 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biphenyl Compounds
• Lignans
• magnolol
• honokiol
• Calcium

Topics

• Animals
• Biphenyl Compounds (pharmacology)
• Calcium (metabolism)
• Cell Line, Tumor
• Cells, Cultured
• Cerebral Cortex (drug effects, metabolism)
• Female
• Humans
• Lignans
• Neuroblastoma (metabolism)
• Neurons (drug effects, metabolism)
• Pregnancy
• Rats
• Rats, Sprague-Dawley