Effect of synthetic oligopeptides on osteoporosis.

Abstract

The objective of this study was to establish the solution method of GHRPS, the synthetic oligopeptides Tyr-Gly-Gly-Phe-Met-NH2, Tyr-Gly-Gly-Phe-Met-OH, Tyr-Gly-Gly-Phe-Leu-NH2, Tyr-Gly-Gly-Phe-Leu-OH, Tyr-Gly-Gly-Phe-Gly-NH2, and Tyr-Gly-Gly-Phe-Gly-OH, to verify their effect on osteoporosis. Male ICR mice (20 +/- 2 g) were used. The intramuscular injection dose of 6.3 mg/kg prednisone induced a significant decrease of body and femur weight of the animals. The subcutaneous injection dose of 18 microg/kg synthetic peptide was not effective to prevent the decrease of body and femur weight of the animals. The subcutaneous injection dose of 6.3 mg/kg prednisone elicited a decrease in content of femur calcium and in the level of serum calcium of the animals. The subcutaneous injection dose of 18 microg/kg Tyr-Gly-Gly-Phe-Leu-NH2, or Tyr-Gly-Gly-Phe-Leu-OH, or Tyr-Gly-Gly-Phe-Gly-NH2, significantly increased the content of femur calcium and decreased the level of serum calcium of the animals. It was also observed that the subcutaneous injection dose of 18 microg/kg Tyr-Gly-Gly-Phe-Gly-OH, Tyr-Gly-Gly-Phe-Leu-OH, Tyr-Gly-Gly-Phe-Met-OH, Tyr-Gly-Gly-Phe-Met-NH2 significantly increased the content of femur phosphorous and decreased the activity of ALP of the animals.

• Authors: Cui Weina, Wang Chao, Zhao Ming, Peng Shiqi
• Journal: Preparative biochemistry & biotechnology (Prep Biochem Biotechnol) Vol. 33 Issue 3 Pg. 217-31 (Aug 2003) ISSN: 1082-6068 [Print] England
• PMID: 12916813 (Publication Type: Journal Article)

Chemical References

• Oligopeptides
• Calcium

Topics

• Amino Acid Sequence
• Animals
• Body Weight
• Calcium (blood, metabolism)
• Femur (drug effects, metabolism)
• Male
• Mice
• Mice, Inbred ICR
• Oligopeptides (chemical synthesis, chemistry, therapeutic use)
• Organ Size
• Osteoporosis (blood, drug therapy)
• Rats