Anti-obesity effect of Dioscorea nipponica Makino with lipase-inhibitory activity in rodents.

Abstract

In the process of screening for pancreatic lipase inhibitors, which could be used as an anti-obesity measure, the methanol extract of Dioscorea nipponica Makino powder (DP) appeared to have potent inhibitory activity against porcine pancreatic lipase with an IC50 value of 5-10 microg/ml, where the enzyme activity was assayed by using 4-methylumbelliferyl oleate as a substrate. Further purification of active components present in the herb generated dioscin that belongs to the saponin family. Dioscin and its aglycone, diosgenin, both suppressed the time-dependent increase of blood triacylglycerol level when orally injected with corn oil to mice, suggesting their inhibitory potential against fat absorption. Sprague-Dawley rats fed on a high-fat diet containing 5% Dioscorea nipponica Makino and 40% beef tallow gained significantly less body weight and adipose tissue than control animals fed on a high-fat diet alone during an 8-week experimental period (P<0.05).

• Authors: Chong-Suk Kwon, Ho Yong Sohn, Sung Hee Kim, Ji Hyun Kim, Kun Ho Son, Jeong Soon Lee, Jin Kyu Lim, Jong-Sang Kim
• Journal: Bioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 67 Issue 7 Pg. 1451-6 (Jul 2003) ISSN: 0916-8451 [Print] England
• PMID: 12913286 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Obesity Agents
• Dietary Fats
• Emulsions
• Plant Extracts
• Triglycerides
• dioscin
• Lipase
• Diosgenin

Topics

• Adipose Tissue (drug effects)
• Animals
• Anti-Obesity Agents (pharmacology, therapeutic use)
• Body Weight (drug effects)
• Diet
• Dietary Fats (administration & dosage, adverse effects)
• Dioscorea (chemistry)
• Diosgenin (analogs & derivatives, pharmacology)
• Emulsions (administration & dosage)
• Feces (chemistry)
• Lipase (antagonists & inhibitors)
• Male
• Mice
• Obesity (blood, drug therapy, prevention & control)
• Organ Size (drug effects)
• Pancreas (enzymology)
• Plant Extracts (pharmacology, therapeutic use)
• Rats
• Rats, Sprague-Dawley
• Triglycerides (blood)