Expression of CS-1 fibronectin precedes monocyte chemoattractant protein-1 production during elicitation of allergic contact dermatitis.

Abstract

BACKGROUND:

Leucocyte migration within inflammatory skin compartments in allergic contact dermatitis (ACD) is the result of a sophisticated multi-step event where multiple molecules are involved.

OBJECTIVE:

Since non-antigen-specific mechanisms have been described as an early participant in elicitation of ACD, we investigated the kinetics of the expression of monocyte chemoattractant protein-1 (MCP-1/CCL2) and the type of infiltrating cells. We compared the time course production of MCP-1/CCL2 with connecting segment-1 (CS-1) fibronectin and thymus and activation-regulated chemokine (TARC/ CCL17) expression.

METHODS:

Biopsies from 10 individuals challenged in their back with the antigen responsible for their contact dermatitis and an irrelevant antigen were taken at different times and histology, immunohistochemistry for CS-1 fibronectin, TARC/CCL17, CD3, CD68, CXCR3, CCR4 and in situ hybridization for MCP-1/CCL2 were performed.

RESULTS:

At positive antigen stimulated sites expression of MCP-1/CCL2 by basal keratinocytes and isolated cells in dermis started at 10 h. CS-1 fibronectin and TARC/CCL17 expression by blood endothelial cells was found at 2 and 10 h, respectively. This was followed by dermal accumulation of mononuclear cells with a significant increase of CD3+ and CD68+cells. At 48 h, approximately 58% of infiltrating cells were CXCR3+, and 35% CCR4+.

CONCLUSIONS:

We showed evidence of the fact that CS-1 fibronectin expression precedes the production of MCP-1/CCL2 and TARC/CCL17 in the skin of patients with ACD, suggesting that these molecules participate in the early complex process of migrating mononuclear cells during elicitation of ACD.

• Authors: A P Martín, J Gagliardi, C E Baena-Cagnani, Y Eberhard, M Uguccioni, N Gallino, A L Mariani, H M Serra
• Journal: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology (Clin Exp Allergy) Vol. 33 Issue 8 Pg. 1118-24 (Aug 2003) ISSN: 0954-7894 [Print] England
• PMID: 12911787 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CCL17 protein, human
• Carrier Proteins
• Chemokine CCL17
• Chemokine CCL2
• Chemokines, CC
• Oligopeptides
• RNA, Messenger
• glutamyl-isoleucyl-leucyl-aspartyl-valine

Topics

• Adult
• Aged
• Biopsy
• Carrier Proteins (biosynthesis)
• Chemokine CCL17
• Chemokine CCL2 (biosynthesis, genetics)
• Chemokines, CC (biosynthesis)
• Dermatitis, Allergic Contact (immunology, metabolism, pathology)
• Female
• Gene Expression
• Humans
• Immunoenzyme Techniques
• In Situ Hybridization
• Male
• Middle Aged
• Oligopeptides (biosynthesis)
• Patch Tests (methods)
• RNA, Messenger (genetics)
• Skin (immunology, metabolism)