[Differentiation and malignant suppression induced by mouse erythroid differentiation and denucleation factor on mouse erythroleukemia cells].

Abstract

OBJECTIVE:

To investigate the roles of mouse erythroid differentiation and denucleation factor (MEDDF), newly cloned in our laboratory, in erythroid terminal differentiation.

METHODS:

Mouse erythroleukemia cells (MEL) were transfected with eukaryotic expression plasmid pcDNA-MEDDF. The changes of cell growth rate, mitotic index and colony-forming rate in semi-solid medium were investigated. The expressions of c-myc and beta-globin genes were analysed by semi-quantitative RT-PCR.

RESULTS:

MEL cells transfected with pcDNA-MEDDF showed significant lower growth rate, mitotic index, and colony-forming rate in semisolid medium(P < 0.01). The percentage of benzidine-positive cells was 32.8% after transfection. The expression of beta-globin in cells transfected with pcDNA-MEDDF was 3.43 times higher than that of control (MEL transfected with blank vector, pcDNA3.1), and the expression of c-myc was decreased by 66.3%.

CONCLUSIONS:

MEDDF can induce differentiation of MEL cell, and suppress its malignancy likely.

• Authors: Q Zhao, Y H Ge, J P Zhou, J Ma, K Q Chen, S P Xue, D S Han
• Journal: Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae (Zhongguo Yi Xue Ke Xue Yuan Xue Bao) Vol. 23 Issue 1 Pg. 32-5 (Feb 2001) ISSN: 1000-503X [Print] China
• PMID: 12905814 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Activins
• Globins
• Inhibin-beta Subunits

Topics

• Activins (genetics, physiology)
• Animals
• Cell Differentiation
• Gene Expression
• Genes, myc (genetics)
• Globins (genetics)
• Inhibin-beta Subunits (genetics, physiology)
• Leukemia, Erythroblastic, Acute (genetics, pathology)
• Mice
• Transfection
• Tumor Cells, Cultured