Abstract | OBJECTIVE: METHODS: Human neuroblastoma SH-SY5Y Cells were exposed to sodium azide, then mitochondrial complex IV activity was assayed by microassay method; cell viability was measured by Thiazolyl blue(MTT) method; mitochondrial membrane potential ( MMP) was detected by confocal microscopy and flow cytometry. RESULTS: Cultured SH-SY5Y cells were exposed to 16-64 mmol/L sodium azide for 1 hour, the mitochondrial complex IV activity decreased dose-dependently. MTT absorbance decreased does- and time-dependently in cultured nerve cells treated by 16-128 mmol/L sodium azide for 1-8 hours. After the treatment of 16 mmol/L sodium azide for 1 hour, both the fluorescence intensity of MMP and normal cell events reduced. Decrease of MMP was significant especially in cell processes. CONCLUSION:
Sodium azide induced the impairment of mitochondrial energy synthesis in the cultured nerve cells which is an important cause in cell death.
|
Authors | L Zhang, L Li, L Ban, W An, S Liu, X Li, B Xue, Y Xu |
Journal | Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae
(Zhongguo Yi Xue Ke Xue Yuan Xue Bao)
Vol. 22
Issue 5
Pg. 436-9
(Oct 2000)
ISSN: 1000-503X [Print] China |
PMID | 12903423
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Sodium Azide
- Electron Transport Complex IV
|
Topics |
- Cell Death
(drug effects)
- Electron Transport Complex IV
(drug effects, metabolism)
- Humans
- Membrane Potentials
(drug effects)
- Mitochondria
(drug effects, physiology)
- Neuroblastoma
(pathology, physiopathology)
- Sodium Azide
(pharmacology)
- Tumor Cells, Cultured
|