Abstract | BACKGROUND:
Fc epsilon RI expressed on the surface of human epidermal Langerhans' cells facilitates uptake of IgE-associated allergens and plays a pivotal role in the pathogenesis of atopic dermatitis. Seminal results from studies investigating Langerhans' cell Fc epsilon RI in skin biopsy sections or epidermal cell suspensions demonstrate the highest receptor expression in lesional skin of patients with active atopic dermatitis. OBJECTIVE: We sought to investigate and localize Fc epsilon RI expression on Langerhans' cells within a minimally disturbed tissue environment in clinically uninvolved skin and to compare receptor expression between healthy donors and patients with atopic dermatitis or other allergic diseases. METHODS: Intact epidermal sheets from skin suction blisters, immunofluorescently stained with Langerhans' cell markers and anti- Fc epsilon RI alpha (mAbs 15E5 and 22E7) or anti-IgE, were examined by means of confocal microscopy. Samples incubated with anti- Fc epsilon RI alpha before or after cell fixation-permeabilization were compared to discriminate between cytoplasmic and membrane localization. RESULTS: CONCLUSION: In clinically uninvolved skin, Langerhans' cell-surface Fc epsilon RI expression is not only linked to atopic dermatitis but is also generally associated with allergic disease. This supports the concept of a systemic regulatory mechanism associated with active allergic disease, which is further aggravated by local inflammation in atopic skin lesions.
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Authors | Amanda E Semper, Kyle Heron, Alexander C s Woollard, Jarema P Kochan, Peter S Friedmann, Martin K Church, Ilona G Reischl |
Journal | The Journal of allergy and clinical immunology
(J Allergy Clin Immunol)
Vol. 112
Issue 2
Pg. 411-9
(Aug 2003)
ISSN: 0091-6749 [Print] United States |
PMID | 12897750
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Receptors, IgE
- Immunoglobulin E
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Topics |
- Asthma
(etiology, pathology, physiopathology)
- Cell Membrane
(metabolism)
- Dermatitis, Atopic
(pathology, physiopathology)
- Epidermis
(metabolism, pathology)
- Fluorescent Antibody Technique
- Humans
- Hypersensitivity
(complications)
- Immunoglobulin E
(metabolism)
- Langerhans Cells
(metabolism)
- Receptors, IgE
(metabolism)
- Rhinitis
(pathology, physiopathology)
- Skin
(metabolism, pathology)
- Staining and Labeling
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