Immunocytochemical analysis of blood lymphocytes in uveitis.

Abstract

We studied the surface expression of activation markers IL2-R, HLA-DR and CD45-RO on peripheral T-lymphocytes in two groups of patients (n = 26) with idiopathic uveoretinitis, compared with controls. Thirteen patients were analysed by alkaline phosphatase anti-alkaline phosphatase (APAAP) immunocytochemistry, which demonstrated a significant rise in expression of HLA-DR and IL2-R surface markers. Flow cytometric analysis was performed on a further 13 patients, which confirmed a significant rise in IL2-R expression in uveitis patients. Within this group systemic activation was confined to patients with idiopathic retinal vasculitis. Dual flow cytometry confirmed a CD4+,IL2--R+ T--lymphocyte phenotype. A further 4 patients with retinal vasculitis who had been treated with cyclosporin A demonstrated a 32% reduction in IL2-R expression over a 3-month period. Analysis of CD45-RO and CD5+ cells was found to be uninformative in this study. We have demonstrated activated peripheral lymphocytes in patients predominantly with retinal vasculitis, the significance of which is discussed.

• Authors: A D Dick, Y F Cheng, A T Purdie, J Liversidge, J V Forrester
• Journal: Eye (London, England) (Eye (Lond)) Vol. 6 ( Pt 6) Pg. 643-7 ( 1992) ISSN: 0950-222X [Print] England
• PMID: 1289145 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• HLA-DR Antigens
• Receptors, Interleukin-2
• Cyclosporine
• Leukocyte Common Antigens

Topics

• Biomarkers (analysis)
• Cyclosporine (therapeutic use)
• Flow Cytometry
• HLA-DR Antigens (analysis)
• Humans
• Immunoenzyme Techniques
• Leukocyte Common Antigens (analysis)
• Lymphocyte Activation (immunology)
• Receptors, Interleukin-2 (analysis)
• Retinal Vessels
• Retinitis (drug therapy, immunology)
• T-Lymphocytes (immunology)
• Uveitis, Posterior (immunology)