Differential binding of Sin3 interacting repressor domains to the PAH2 domain of Sin3A.

Abstract	The Sin3 interacting domain (SID), originally described in the Mad family of repressors, is a novel transcriptional repressor domain that binds the PAH2 domain of corepressors Sin3A and Sin3B with high affinities. The conserved SID-like domains are reportedly present in five KLF proteins. However, the KLF SIDs and the Mad SIDs can be classified into two subtypes according to sequence similarity. Here, we report the finding from computational and experimental studies that the two subtypes of SID domains bind differentially to Sin3A. This finding offers insights into a mechanism of cell growth regulation by interactions of different subtypes of SID-containing repressor proteins with Sin3. It also provides the structural basis for developing selective modulators of Sin3.
Authors	Yuan-Ping Pang, Ganesh A Kumar, Jin-San Zhang, Raul Urrutia
Journal	FEBS letters (FEBS Lett) Vol. 548 Issue 1-3 Pg. 108-12 (Jul 31 2003) ISSN: 0014-5793 [Print] England
PMID	12885416 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References	Apoptosis Regulatory Proteins Cell Cycle Proteins KLF11 protein, human KLF13 protein, human Kruppel-Like Transcription Factors MAD1 protein, S cerevisiae MAD1L1 protein, human Nuclear Proteins Phosphoproteins Repressor Proteins SIN3 protein, S cerevisiae SIN3A transcription factor SIN3B protein, human Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors Histone Deacetylases Sin3 Histone Deacetylase and Corepressor Complex
Topics	Apoptosis Regulatory Proteins Binding Sites Cell Cycle Proteins Computer Simulation Conserved Sequence Histone Deacetylases Kruppel-Like Transcription Factors Models, Molecular Mutagenesis, Site-Directed Mutation, Missense Nuclear Proteins Phosphoproteins (chemistry, metabolism) Protein Binding Protein Structure, Tertiary Repressor Proteins (chemistry, metabolism) Saccharomyces cerevisiae Proteins Sin3 Histone Deacetylase and Corepressor Complex Trans-Activators (chemistry, genetics, metabolism) Transcription Factors (chemistry, metabolism)

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