Abstract | PURPOSE: METHODS: The complete coding sequence of the canine CNTFRalpha cDNA was cloned, and radiation hybrid (RH) mapping was used to determine the chromosomal localization of the gene. CNTFRalpha mRNA expression in retina and other tissues was examined by reverse transcription-polymerase chain reaction. The cellular distribution of CNTFRalpha in the canine retina was studied by in situ hybridization and immunocytochemistry. RESULTS: Canine CNTFRalpha shares a high degree of homology with the human, mouse, and rat coding sequences, both at the nucleotide and amino acid level, but has lower homology with the chicken. CNTFRalpha was RH mapped to CFA 11 (Canis familiaris autosome 11) in the dog, a region showing homology to the short arm of human chromosome 9 (9p13). The gene is transcribed in retina, brain, spleen, lung, liver, and kidney. In the retina, CNTFRalpha was highly expressed by photoreceptors, but both the transcript and protein were also found in the RPE, inner nuclear layer, and ganglion cells. CONCLUSIONS:
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Authors | William A Beltran, Qi Zhang, James W Kijas, Danian Gu, Hermann Rohrer, Julie A Jordan, Gustavo D Aguirre |
Journal | Investigative ophthalmology & visual science
(Invest Ophthalmol Vis Sci)
Vol. 44
Issue 8
Pg. 3642-9
(Aug 2003)
ISSN: 0146-0404 [Print] United States |
PMID | 12882818
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- RNA, Messenger
- Receptor, Ciliary Neurotrophic Factor
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Topics |
- Amino Acid Sequence
- Animals
- Cloning, Molecular
- Dogs
(genetics)
- Gene Expression
- Immunoblotting
(veterinary)
- Immunoenzyme Techniques
(veterinary)
- In Situ Hybridization
(veterinary)
- Male
- Molecular Sequence Data
- RNA, Messenger
(metabolism)
- Radiation Hybrid Mapping
(veterinary)
- Receptor, Ciliary Neurotrophic Factor
(genetics)
- Retina
(metabolism)
- Sequence Homology, Amino Acid
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