The modulatory effects of a hydro-alcoholic extract of drumsticks of Moringa oliefera
Lam at doses of 125 mg/kg bodyweight and 250 mg/ kg
body weight for 7 and 14 days, respectively, were investigated with reference to
drug metabolising Phase I (
Cytochrome b(5) and
Cytochrome p(450) ) and Phase II (
Glutathione-S- transferase)
enzymes,
anti-oxidant enzymes,
glutathione content and lipid peroxidation in the liver of 6-8 week old female Swiss albino mice. Further, the chemopreventive efficacy of the extract was evaluated in a two stage model of 7,12 - dimethylbenz(a)
anthracene induced skin papillomagenesis. Significant increase (p<0.05 to p<0.01) in the activities of hepatic
cytochrome b(5),
cytochrome p(450),
catalase,
glutathione peroxidase ( GPx ),
glutathione reductase (GR),
acid soluble sulfhydryl content (-SH ) and a significant decrease ( p<0.01 ) in the hepatic MDA level were observed at both dose levels of treatment when compared with the control values.
Glutathione-S- transferase ( GST )activity was found to be significantly increased (p<0.01 ) only at the higher dose level. Butylated hydroxyanisol (
BHA ) fed at a dose of 0.75% in the diet for 7 and 14 days (positive control ) caused a significant increase (p<0.05 to p<0.01) in the levels of hepatic phase I and phase II
enzymes,
anti- oxidant enzymes,
glutathione content and a decrease in lipid peroxidation. The skin papillomagenesis studies demonstrated a significant decrease (p<0.05 ) in the percentage of mice with
papillomas, average number of
papillomas per mouse and
papillomas per
papilloma bearing mouse when the animals received a topical application of the extract at a dose of 5mg/ kg
body weight in the peri-initiation phase 7 days before and 7 days after DMBA application, Group II ), promotional phase (from the day of
croton oil application and continued till the end of the experiment, Group III ) and both peri and post initiation stages (from 7 days prior to DMBA application and continued till the end of the experiment, Group IV) compared to the control group (Group I ). The percentage inhibition of
tumor multiplicity has been recorded to be 27, 72, and 81 in Groups II, III, and IV, respectively. These findings are suggestive of a possible chemopreventive potential of Moringa oliefera drumstick extract against chemical
carcinogenesis.