Abstract | BACKGROUND: METHODS: Left lungs of male Long-Evans rats were rendered ischemic for 90 minutes and reperfused for up to 4 hours. Treated animals received FP-15 (a water-soluble iron containing metalloporphyrin that acts as a peroxynitrite decomposition catalyst). Injury was quantitated in terms of tissue neutrophil accumulation ( myeloperoxidase content) and vascular permeability ((125)I bovine serum albumin [BSA] extravasation) and bronchoalveolar lavage cytokine, transcriptional factor and leukocyte content. Separate tissue samples were processed for immunohistology and nuclear protein analysis. RESULTS: Lung vascular permeability was reduced in treated animals by 61% compared with control animals (p < 0.005). The protective effects of enhanced peroxynitrite decomposition correlated with a 72% reduction in tissue myeloperoxidase content (p < 0.001) and marked reductions in brochoalveolar lavage leukocyte accumulation. This correlated positively with the diminished expression of pro-inflammatory chemokines and nuclear transcription factors. CONCLUSIONS:
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Authors | Babu V Naidu, Charles Fraga, Andrew L Salzman, Csaba Szabo, Edward D Verrier, Michael S Mulligan |
Journal | The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation
(J Heart Lung Transplant)
Vol. 22
Issue 7
Pg. 784-93
(Jul 2003)
ISSN: 1053-2498 [Print] United States |
PMID | 12873547
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Chemokines
- Inflammation Mediators
- NF-kappa B
- Reactive Nitrogen Species
- Transcription Factor AP-1
- Peroxynitrous Acid
- 3-nitrotyrosine
- Tyrosine
- Peroxidase
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Topics |
- Animals
- Bronchoalveolar Lavage Fluid
(cytology)
- Capillary Permeability
(drug effects)
- Chemokines
(metabolism)
- Disease Models, Animal
- Immunohistochemistry
- Inflammation Mediators
(metabolism)
- Macrophages, Alveolar
(drug effects, metabolism)
- Male
- Models, Cardiovascular
- NF-kappa B
(drug effects, metabolism)
- Peroxidase
(drug effects, metabolism)
- Peroxynitrous Acid
(pharmacology)
- Rats
- Rats, Long-Evans
- Reactive Nitrogen Species
(physiology)
- Reperfusion Injury
(metabolism, physiopathology)
- Respiratory Distress Syndrome
(metabolism, physiopathology)
- Severity of Illness Index
- Statistics as Topic
- Transcription Factor AP-1
(drug effects, metabolism)
- Transcriptional Activation
(drug effects)
- Tyrosine
(analogs & derivatives, drug effects, metabolism)
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