Abstract |
Eurystatins A and B, which are produced by Streptomyces eurythermus R353-21, potently inhibited Flavobacterium prolyl endopeptidase (PED) with IC50 values of 0.004 and 0.002 micrograms/ml, respectively, while no inhibition was observed against another 5 proteases, even at 100 micrograms/ml. The protective effect of eurystatins A and B against scopolamine (3 mg/kg, i.p.)-induced amnesia in rats was evaluated by the step-through one-trial passive avoidance method. When administered i.p. 30 min prior to the acquisition trial, both eurystatins A, at 2-8 mg/kg, and B, at 4-8 mg/kg, significantly protected rats from the amnesic effect of scopolamine without behavioral side effects.
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Authors | H Kamei, T Ueki, Y Obi, Y Fukagawa, T Oki |
Journal | Japanese journal of pharmacology
(Jpn J Pharmacol)
Vol. 60
Issue 4
Pg. 377-80
(Dec 1992)
ISSN: 0021-5198 [Print] Japan |
PMID | 1287273
(Publication Type: Journal Article)
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Chemical References |
- Peptides, Cyclic
- Serine Proteinase Inhibitors
- eurystatin A
- eurystatin B
- Scopolamine
- Serine Endopeptidases
- Prolyl Oligopeptidases
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Topics |
- Amino Acid Sequence
- Amnesia
(chemically induced)
- Animals
- Avoidance Learning
(drug effects)
- Dose-Response Relationship, Drug
- Flavobacterium
(drug effects, enzymology)
- Molecular Sequence Data
- Peptides, Cyclic
(pharmacology)
- Prolyl Oligopeptidases
- Rats
- Scopolamine
(antagonists & inhibitors, pharmacology)
- Serine Endopeptidases
(metabolism)
- Serine Proteinase Inhibitors
(pharmacology)
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