Association of muscle glycogen synthase polymorphism with insulin resistance in type 2 diabetic patients.

Abstract

The aim of the present study is to investigate whether Met416Val (M416V) polymorphism of glycogen synthase (GYS1) gene is associated with insulin resistance in type 2 diabetes. In 100 type 2 diabetic subjects (66 men and 34 women), the M416V polymorphism of GYS1 gene was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) as previously reported, and insulin resistance was assessed by euglycemic hyperinsulinemic clamp represented as M/I value, the mean of glucose infusion rate (M value) adjusted by steady state plasma insulin level. The means of age and body mass index (BMI) of the subjects were 53.1+/-11.6 (SD) years and 23.3+/-3.5 kg/m2. The allele frequencies of M416V polymorphism were 82.0% for MM, 16.0% for MV, and 2.0% for VV, and subjects were subsequently divided into V(+) group (n=18) and V(-) group (n=82) according to the presence or absence of V allele. There were no significant differences in age, BMI, blood pressure, fasting plasma glucose or insulin levels or glycosylated hemoglobin (HbA1c) levels between the V(+) and V(-) groups. No significant differences in either M or M/I value were found between the V(+) and V(-) groups (M value, 5.06+/-2.20 v 5.12+/-2.04 mg x kg(-1) x min(-1), P=.841; M/I value, 5.24+/-3.07 v 5.39+/-2.87 mg x kg(-1) x min(-1) x mU(-1) x L, P=.576). BMI showed the strongest independent contribution to M/I value, but the presence of V allele did not in multiple regression analysis. In conclusion, the M416V polymorphism of GYS1 gene is not associated with insulin resistance in type 2 diabetes.

• Authors: Koka Motoyama, Masanori Emoto, Hideki Tahara, Miyoko Komatsu, Tetsuo Shoji, Masaaki Inaba, Yoshiki Nishizawa
• Journal: Metabolism: clinical and experimental (Metabolism) Vol. 52 Issue 7 Pg. 895-9 (Jul 2003) ISSN: 0026-0495 [Print] United States
• PMID: 12870167 (Publication Type: Journal Article)

Chemical References

• Blood Glucose
• Glycated Hemoglobin A
• Insulin
• Glycogen Synthase

Topics

• Adult
• Alleles
• Blood Glucose (analysis)
• Body Mass Index
• Diabetes Mellitus, Type 2 (genetics)
• Female
• Gene Frequency
• Glucose Clamp Technique
• Glycated Hemoglobin (analysis)
• Glycogen Synthase (genetics)
• Humans
• Insulin (blood)
• Insulin Resistance (genetics)
• Male
• Middle Aged
• Muscle, Skeletal (enzymology)
• Polymorphism, Genetic
• Regression Analysis