Abstract |
To determine the exact role of TGF-alpha in glial activation after traumatic brain injury, we investigated the astroglial and microglial responses after cortical stab wound injury in TGF-alpha overexpressing mice. Adult male B6D2-TgN (MMTVTGFA) 29RjC transgenic mice were used for the subjects. This transgenic line carries a TGF-alpha cDNA under the control of the dexamethasone-inducible MMTV promoter. Thus, exogenous administration of dexamethasone induces TGF-alpha overexpression. Male B6D2F1/J mice at the same age served as wild-type animals. After the cortical stab wound injury, expression of glial fibrillary acidic protein, CD-11b and interleukine-6 were investigated immunohistochemically. The results indicate that TGF-alpha might affect astrocytic hypertrophy without affecting microgliosis not only in the normal condition, but also in the pathological condition. Moreover, overexpression of TGF-alpha induced obvious expression of IL-6 around the lesion. This fact might indicate possible role of TGF-alpha in affecting neuronal function.
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Authors | Mitsuo Isono, Makoto Goda, Hidenori Kobayashi, Jan Lian Wu |
Journal | Neurological research
(Neurol Res)
Vol. 25
Issue 5
Pg. 546-50
(Jul 2003)
ISSN: 0161-6412 [Print] England |
PMID | 12866207
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CD11b Antigen
- Glial Fibrillary Acidic Protein
- Interleukin-6
- Transforming Growth Factor alpha
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Topics |
- Animals
- Astrocytes
(chemistry, pathology)
- Brain Injuries
(pathology, physiopathology)
- CD11b Antigen
(analysis)
- Gene Expression
- Glial Fibrillary Acidic Protein
(analysis)
- Hypertrophy
- Interleukin-6
(analysis)
- Male
- Mice
- Mice, Transgenic
- Transforming Growth Factor alpha
(analysis, genetics)
- Wounds, Stab
(pathology, physiopathology)
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