Abstract | BACKGROUND AND OBJECTIVES: MATERIALS AND METHODS: RESULTS: Higher phototoxicity was obtained with higher dye and/or laser doses. Most of the dead cells appeared apoptotic with dye and irradiation doses that induced less than 70% cytotoxicity. In contrast, most of them appeared necrotic with doses that induced 99% cytotoxicity. Cells receiving PDT showed disturbances of mitochondrial trans-membrane potential, although the primary site of ATX-S10(Na) accumulation was in lysosomes. CONCLUSIONS: ATX-S10(Na) has a phototoxic effect on malignant melanoma cells and, therefore, potential as a photosensitizing agent for PDT designed to kill these cells. Apoptotic pathways may be activated via mitochondrial destabilization following the damage of lysosomes by PDT. Further study, including investigation of therapeutic efficacy in vivo, is warranted.
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Authors | Satoshi Nagata, Akira Obana, Yuko Gohto, Susumu Nakajima |
Journal | Lasers in surgery and medicine
(Lasers Surg Med)
Vol. 33
Issue 1
Pg. 64-70
( 2003)
ISSN: 0196-8092 [Print] United States |
PMID | 12866123
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2003 Wiley-Liss, Inc. |
Chemical References |
- ATX S10Na(II)
- Photosensitizing Agents
- Porphyrins
- Surface-Active Agents
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Topics |
- Apoptosis
(drug effects, radiation effects)
- Cell Line, Tumor
- Humans
- In Vitro Techniques
- Melanoma
(drug therapy, ultrastructure)
- Microscopy, Fluorescence
- Necrosis
- Photochemotherapy
- Photosensitizing Agents
(therapeutic use)
- Porphyrins
- Surface-Active Agents
(therapeutic use)
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