Abstract | OBJECTIVES: STUDY DESIGN: Prospective study with synovial fluid sampling from diseased and contralateral unaffected joints at 0, 1.5, and 5 months. SAMPLE POPULATION: Eleven dogs with unilateral CCL deficiency, with unaffected contralateral joints. METHODS:
IGF-I and IGF-II concentrations in synovial fluids were estimated by radioimmunoassay at 0, 1.5, and 5 months; Western ligand blotting was performed for intact IGFBPs at 0, 1.5, 5, and 9 months. Both stifle joints were radiographed at 0, 7, and 13 months. RESULTS: The IGF system is altered after CCL rupture and during development of early OA. Mean IGF-I and IGF-II concentrations in index stifle joints at study entry were 201.6 microg/mL and 345.7 microg/mL, respectively, compared with 57.7 microg/mL and 79.4 microg/mL, respectively, for contralateral joints. Index joint IGF concentrations increased after surgical treatment and then declined, although they remained higher than contralateral joints. Index joints had increases in IGFBP-3 and -4, and a decrease in IGFBP-2 expression compared with contralateral joints. CONCLUSIONS: Although IGF concentrations are increased in canine OA, alterations in IGFBP profiles may limit the tissue availability of IGF. CLINICAL RELEVANCE: Manipulation of the IGF system may provide an opportunity for novel treatments of OA in dogs.
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Authors | Janet K Fernihough, John F Innes, Michael E J Billingham, Jeff M P Holly |
Journal | Veterinary surgery : VS
(Vet Surg)
2003 Jul-Aug
Vol. 32
Issue 4
Pg. 313-23
ISSN: 0161-3499 [Print] United States |
PMID | 12865993
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2003 by The American College of Veterinary Surgeons |
Chemical References |
- Insulin-Like Growth Factor Binding Proteins
- Insulin-Like Growth Factor I
- Insulin-Like Growth Factor II
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Topics |
- Animals
- Anterior Cruciate Ligament
(diagnostic imaging, immunology)
- Anterior Cruciate Ligament Injuries
- Blotting, Western
(veterinary)
- Dog Diseases
(immunology, surgery)
- Dogs
(injuries)
- Injury Severity Score
- Insulin-Like Growth Factor Binding Proteins
(metabolism)
- Insulin-Like Growth Factor I
(metabolism)
- Insulin-Like Growth Factor II
(metabolism)
- Osteoarthritis
(immunology, veterinary)
- Prospective Studies
- Radiography
- Radioimmunoassay
(veterinary)
- Rupture, Spontaneous
(veterinary)
- Stifle
(immunology, injuries)
- Synovial Fluid
(immunology)
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