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Changes in the local regulation of insulin-like growth factors I and II and insulin-like growth factor-binding proteins in osteoarthritis of the canine stifle joint secondary to cruciate ligament rupture.

AbstractOBJECTIVES:
To investigate changes in concentrations of insulin-like growth factors I (IGF-I) and II (IGF-II) and the expression of IGF-binding proteins (IGFBP) in synovial fluids from dogs with naturally occurring osteoarthritis (OA) of the canine stifle joint secondary to cranial cruciate ligament (CCL) rupture.
STUDY DESIGN:
Prospective study with synovial fluid sampling from diseased and contralateral unaffected joints at 0, 1.5, and 5 months.
SAMPLE POPULATION:
Eleven dogs with unilateral CCL deficiency, with unaffected contralateral joints.
METHODS:
IGF-I and IGF-II concentrations in synovial fluids were estimated by radioimmunoassay at 0, 1.5, and 5 months; Western ligand blotting was performed for intact IGFBPs at 0, 1.5, 5, and 9 months. Both stifle joints were radiographed at 0, 7, and 13 months.
RESULTS:
The IGF system is altered after CCL rupture and during development of early OA. Mean IGF-I and IGF-II concentrations in index stifle joints at study entry were 201.6 microg/mL and 345.7 microg/mL, respectively, compared with 57.7 microg/mL and 79.4 microg/mL, respectively, for contralateral joints. Index joint IGF concentrations increased after surgical treatment and then declined, although they remained higher than contralateral joints. Index joints had increases in IGFBP-3 and -4, and a decrease in IGFBP-2 expression compared with contralateral joints.
CONCLUSIONS:
Although IGF concentrations are increased in canine OA, alterations in IGFBP profiles may limit the tissue availability of IGF.
CLINICAL RELEVANCE:
Manipulation of the IGF system may provide an opportunity for novel treatments of OA in dogs.
AuthorsJanet K Fernihough, John F Innes, Michael E J Billingham, Jeff M P Holly
JournalVeterinary surgery : VS (Vet Surg) 2003 Jul-Aug Vol. 32 Issue 4 Pg. 313-23 ISSN: 0161-3499 [Print] United States
PMID12865993 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright 2003 by The American College of Veterinary Surgeons
Chemical References
  • Insulin-Like Growth Factor Binding Proteins
  • Insulin-Like Growth Factor I
  • Insulin-Like Growth Factor II
Topics
  • Animals
  • Anterior Cruciate Ligament (diagnostic imaging, immunology)
  • Anterior Cruciate Ligament Injuries
  • Blotting, Western (veterinary)
  • Dog Diseases (immunology, surgery)
  • Dogs (injuries)
  • Injury Severity Score
  • Insulin-Like Growth Factor Binding Proteins (metabolism)
  • Insulin-Like Growth Factor I (metabolism)
  • Insulin-Like Growth Factor II (metabolism)
  • Osteoarthritis (immunology, veterinary)
  • Prospective Studies
  • Radiography
  • Radioimmunoassay (veterinary)
  • Rupture, Spontaneous (veterinary)
  • Stifle (immunology, injuries)
  • Synovial Fluid (immunology)

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