Urinary tract obstruction impairs urinary concentrating capacity and reabsorption of
sodium. To clarify the molecular mechanisms of these defects, expression levels of renal
sodium transporters were examined in rats with 24-h bilateral
ureteral obstruction (BUO) or at day 3 or 14 after release of BUO (BUO-R). BUO resulted in downregulation of type 3 Na+/H+ exchanger (NHE3) to 41 +/- 14%, type 2 Na-Pi cotransporter (NaPi-2) to 26 +/- 6%, Na-K-
ATPase to 67 +/- 8%, type 1
bumetanide-sensitive Na-K-2Cl cotransporter (BSC-1) to 20 +/- 7%, and
thiazide-sensitive cotransporter (
TSC) to 37 +/- 9%. Immunocytochemistry confirmed downregulation of NHE3, NaPi-2, Na-K-
ATPase, BSC-1, and
TSC. Consistent with this downregulation, BUO-R was associated with
polyuria, reduced urinary osmolality, and increased urinary
sodium and
phosphate excretion. BUO-R for 3 days caused a persistant downregulation of NHE3 to 53 +/- 10%, NaPi-2 to 57 +/- 9%, Na-K-
ATPase to 62 +/- 8%, BSC-1 to 50 +/- 12%, and
TSC to 56 +/- 16%, which was associated with a marked reduction in the net renal reabsorption of
sodium (616 +/- 54 vs. 944 +/- 24 micromol x min-1 x kg-1; P < 0.05) and
phosphate (6.3 +/- 0.9 vs. 13.1 +/- 0.4 micromol x min-1. kg-1; P < 0.05) demonstrating a defect in renal
sodium and
phosphate reabsorption capacity. Moreover, downregulation of Na-K-
ATPase and
TSC persisted in BUO-R for 14 days, whereas NHE3, NaPi-2, and BSC-1 were normalized to control levels. In conclusion, downregulation of renal Na transporters in rats with BUO and release of BUO are likely to contribute to the associated urinary concentrating defect, increased urinary
sodium excretion, and postobstructive
polyuria.